Veernala Induvahi, Roopmani Purandhi, Singh Ruby, Hasan Uzma, Giri Jyotsnendu
Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, Kandi, Telangana, India.
Department of Biotechnology, Indian Institute of Technology, Hyderabad, Kandi, Telangana, India.
Prog Biomater. 2021 Jun;10(2):131-150. doi: 10.1007/s40204-021-00158-3. Epub 2021 Jul 5.
Functional tissue regeneration using synthetic biomaterials requires proliferation and heterotypic differentiation of stem/progenitor cells within a specialized heterogeneous (biophysical-biochemical) microenvironment. The current techniques have limitations to develop synthetic hydrogels, mimicking native extracellular matrix porosity along with heterogeneous microenvironmental cues of matrix mechanics, degradability, microstructure and cell-cell interactions. Here, we have developed a microenvironment modulating system to fabricate in situ porous hydrogel matrix with two or more distinct tailored microenvironmental niches within microbeads and the hydrogel matrix for multicellular tissue regeneration. Electrosprayed pectin-gelatin blended microbeads and crosslinked alginate hydrogel system help to tailor microenvironmental niches of encapsulated cells where two different cells are surrounded by a specific microenvironment. The effect of different microenvironmental parameters associated with the microbead/hydrogel matrix was evaluated using human umbilical-cord mesenchymal stem cells (hUCMSCs). The osteogenic differentiation of hUCMSCs in the hydrogel matrix was evaluated for bone tissue regeneration. This will be the first report on microenvironment modulating microbead-hydrogel system to encapsulate two/more types of cells in a hydrogel, where each cell is surrounded with distinct niches for heterogeneous tissue regeneration.
使用合成生物材料进行功能性组织再生需要干细胞/祖细胞在特殊的异质（生物物理 - 生化）微环境中增殖和异型分化。目前的技术在开发合成水凝胶方面存在局限性，难以模拟天然细胞外基质的孔隙率以及基质力学、降解性、微观结构和细胞间相互作用等异质微环境线索。在此，我们开发了一种微环境调节系统，用于制造原位多孔水凝胶基质，该基质在微珠和水凝胶基质内具有两个或更多个不同的定制微环境龛，用于多细胞组织再生。电喷雾果胶 - 明胶混合微珠和交联藻酸盐水凝胶系统有助于定制被封装细胞的微环境龛，其中两种不同的细胞被特定的微环境所包围。使用人脐带间充质干细胞（hUCMSCs）评估了与微珠/水凝胶基质相关的不同微环境参数的影响。评估了hUCMSCs在水凝胶基质中的成骨分化以用于骨组织再生。这将是关于微环境调节微珠 - 水凝胶系统在水凝胶中封装两种或更多种类型细胞的第一份报告，其中每个细胞被不同的龛包围以用于异质组织再生。
