Yu Baozhong, Zhang Jiandong, Sun Zejia, Cao Peng, Zheng Xiang, Gao Zihao, Cao Haoyuan, Zhang Feilong, Wang Wei
Department of Urology, Affiliated Beijing Chaoyang Hospital of Capital Medical University.
Capital Medical University, Beijing, China.
Medicine (Baltimore). 2021 Jan 8;100(1):e24257. doi: 10.1097/MD.0000000000024257.
Renal cell carcinoma (RCC) accounts for 2% to 3% of all human malignancies and is the 9th most common malignancy in Western countries. Due to the development of surgical procedures and the use of novel drugs, survival has been significantly prolonged. However, current challenges include how to diagnose RCC earlier and how to overcome drug resistance. Methods: We explored the relationship between the transcription level of IFI16 and clinical data in RCC through various online databases, including ONCOMINE, GEPIA, HPA, Timer and COEXPEDIA.
In comparison with corresponding normal tissues, IFI16 mRNA expression levels were higher in kidney renal clear cell carcinoma (KIRC) and kidney renal papillary cell carcinoma (KIRP) tissues. In KIRC, the higher expression of IFI16 was associated with lower overall survival (P = .037). In KIRP, the higher expression IFI16 was associated with lower disease-free survival and overall survival (P = .037 and P = .011). In contrast, the IFI16 expression was negatively correlated with tumor purity in kidney chromophobe, KIRC and KIRP (all P < .05). In KIRC and KIRP, the expression of IFI16 was positively correlated with tumor-infiltrating immune cells (TIICs) (all P < .05), except macrophages in KIRP. In KIRC, the main TIICs were B cells, CD4+T cells, neutrophils, and dendritic cells, while the main TIICs in the high amplification state were macrophage (all P < .0001). Functional enrichment analysis by gene ontology and Kyoto Encyclopedia of Genes and Genomes highlighted enrichment of neutrophil degranulation, phagocytosis and vesicle-mediated transport regulation, and pathways including tuberculosis, toxoplasmosis, phagosome, leishmaniasis, and Fc gamma R-mediated.
IFI16 is overexpressed in RCC and may be an important oncogene in the progression of kidney. In addition, IFI16 may a marker for RCC diagnosis and prognosis, which may be related to immune infiltration.
肾细胞癌（RCC）占所有人类恶性肿瘤的2%至3%，是西方国家第九大常见恶性肿瘤。由于外科手术的发展和新型药物的使用，患者生存期显著延长。然而，当前的挑战包括如何更早地诊断RCC以及如何克服耐药性。方法：我们通过各种在线数据库，包括ONCOMINE、GEPIA、HPA、Timer和COEXPEDIA，探索了IFI16转录水平与RCC临床数据之间的关系。
与相应的正常组织相比，IFI16 mRNA表达水平在肾透明细胞癌（KIRC）和肾乳头状细胞癌（KIRP）组织中较高。在KIRC中，IFI16的高表达与较低的总生存期相关（P = 0.037）。在KIRP中，IFI16的高表达与较低的无病生存期和总生存期相关（P = 0.037和P = 0.011）。相反，在肾嫌色细胞癌、KIRC和KIRP中，IFI16表达与肿瘤纯度呈负相关（所有P < 0.05）。在KIRC和KIRP中，IFI16的表达与肿瘤浸润免疫细胞（TIICs）呈正相关（所有P < 0.05），KIRP中的巨噬细胞除外。在KIRC中，主要的TIICs是B细胞、CD4 + T细胞、中性粒细胞和树突状细胞，而处于高扩增状态的主要TIICs是巨噬细胞（所有P < 0.0001）。基因本体论和京都基因与基因组百科全书的功能富集分析突出了中性粒细胞脱颗粒、吞噬作用和囊泡介导的转运调节的富集，以及包括结核病、弓形虫病、吞噬体、利什曼病和FcγR介导的途径。
IFI16在RCC中过表达，可能是肾脏进展中的一个重要癌基因。此外，IFI16可能是RCC诊断和预后的一个标志物，这可能与免疫浸润有关。
