Khanal Vivek, Wells Harrington, Ali Akhtar
Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA.
Pathogens. 2021 Jan 8;10(1):53. doi: 10.3390/pathogens10010053.
Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016-2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.
有关感染农作物的病毒的田间信息对于了解它们对植物造成的影响的严重程度至关重要。为了确定葫芦科病毒的状况，于2016年至2018年连续三年在俄克拉荷马州的不同农业区进行了调查。从11个县的有症状和无症状的葫芦科植物中随机采集了来自90多个田地的总共1331个叶片样本。所有样本都用斑点免疫结合测定法（DIBA）针对10种已知病毒的抗血清进行了检测。感染了木瓜环斑病毒（PRSV-W）、西瓜花叶病毒（WMV）、西葫芦黄花叶病毒（ZYMV）和葫芦科蚜虫传播黄化病毒（CABYV）的样本也通过RT-PCR进行了检测。在这10种病毒中，PRSV-W分布最广，总体患病率为59.1%，存在于所有11个县，其次是ZYMV（27.6%），存在于10个县，WMV（20.7%），存在于7个县，而其余病毒则零星出现，发病率较低。大约42%的感染样本呈阳性，感染一种以上病毒表明混合感染的比例很高。CABYV首次在俄克拉荷马州被检测到，对来自美国的一株CABYV分离株（BL-4）的首个完整基因组序列进行的系统发育分析表明，它与亚洲分离株关系密切。
