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一条途径，两种环状非核糖体五肽：来自CA-170360的BE-18257抗生素和戊胺霉素的异源表达

One Pathway, Two Cyclic Non-Ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from CA-170360.

作者信息

Román-Hurtado Fernando, Sánchez-Hidalgo Marina, Martín Jesús, Ortiz-López Francisco Javier, Carretero-Molina Daniel, Reyes Fernando, Genilloud Olga

机构信息

Fundación MEDINA, Avenida del Conocimiento 34, 18016 Granada, Spain.

出版信息

Microorganisms. 2021 Jan 8;9(1):135. doi: 10.3390/microorganisms9010135.

DOI:10.3390/microorganisms9010135
PMID:33430167
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7827011/
Abstract

The strain CA-170360 produces the cyclic pentapeptides pentaminomycins A-H and BE-18257 A-C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the non-ribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.

摘要

菌株CA - 170360产生环五肽戊胺霉素A - H和BE - 18257 A - C，这两类环肽由同一生物合成基因簇中串联编码的两个非核糖体肽合成酶合成。在这项工作中，我们克隆并证实了该生物合成基因簇的异源表达，表明该基因簇中存在的每个非核糖体肽合成酶都参与了每组环肽的生物合成。此外，我们还讨论了一种属于青霉素结合蛋白家族的独立酶在肽的释放和大环化过程中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/9b509d2cb194/microorganisms-09-00135-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/4c78ba50a6f4/microorganisms-09-00135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/f3f5d62b0346/microorganisms-09-00135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/351453996944/microorganisms-09-00135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/dde231bd1fe6/microorganisms-09-00135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/f30106732e4a/microorganisms-09-00135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/3df04cab4fb0/microorganisms-09-00135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/b783043ce2af/microorganisms-09-00135-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/9b509d2cb194/microorganisms-09-00135-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/4c78ba50a6f4/microorganisms-09-00135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/f3f5d62b0346/microorganisms-09-00135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/351453996944/microorganisms-09-00135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/dde231bd1fe6/microorganisms-09-00135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/f30106732e4a/microorganisms-09-00135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/3df04cab4fb0/microorganisms-09-00135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/b783043ce2af/microorganisms-09-00135-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7cf7/7827011/9b509d2cb194/microorganisms-09-00135-g008.jpg

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