Department of Biotechnology and Life Sciences, University of Insubria, DBSV, Via J.H. Dunant 3, 21100 Varese, Italy.
Department of Surgical Sciences, University of Cagliari, 09100 Cagliari, Italy.
Int J Mol Sci. 2021 Jan 7;22(2):526. doi: 10.3390/ijms22020526.
Several types of 3-dimensional (3D) biological matrices are employed for clinical and surgical applications, but few indications are available to guide surgeons in the choice among these materials. Here we compare the in vitro growth of human primary fibroblasts on different biological matrices commonly used for clinical and surgical applications and the activation of specific molecular pathways over 30 days of growth. Morphological analyses by Scanning Electron Microscopy and proliferation curves showed that fibroblasts have different ability to attach and proliferate on the different biological matrices. They activated similar gene expression programs, reducing the expression of collagen genes and myofibroblast differentiation markers compared to fibroblasts grown in 2D. However, differences among 3D matrices were observed in the expression of specific metalloproteinases and interleukin-6. Indeed, cell proliferation and expression of matrix degrading enzymes occur in the initial steps of interaction between fibroblast and the investigated meshes, whereas collagen and interleukin-6 expression appear to start later. The data reported here highlight features of fibroblasts grown on different 3D biological matrices and warrant further studies to understand how these findings may be used to help the clinicians choose the correct material for specific applications.
目前有几种类型的三维（3D）生物基质被应用于临床和外科应用，但很少有适应症可以指导外科医生在这些材料之间进行选择。在这里，我们比较了人类原代成纤维细胞在临床上常用的几种生物基质上的体外生长情况，并在 30 天的生长过程中比较了特定分子通路的激活情况。扫描电子显微镜的形态分析和增殖曲线表明，成纤维细胞在不同的生物基质上附着和增殖的能力不同。与在 2D 中生长的成纤维细胞相比，它们激活了相似的基因表达程序，降低了胶原蛋白基因和肌成纤维细胞分化标志物的表达。然而，在特定金属蛋白酶和白细胞介素-6 的表达方面，3D 基质之间存在差异。事实上，细胞增殖和基质降解酶的表达发生在成纤维细胞与所研究的网片相互作用的初始步骤中，而胶原蛋白和白细胞介素-6 的表达似乎较晚开始。这里报道的数据突出了不同 3D 生物基质上生长的成纤维细胞的特征，并需要进一步的研究来了解如何利用这些发现来帮助临床医生为特定应用选择正确的材料。
