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从黎巴嫩野生虎眼万年青中提取的果聚糖的抗氧化活性和生物相容性

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon.

作者信息

Medlej Mohammad Kazem, Batoul Cherri, Olleik Hamza, Li Suming, Hijazi Akram, Nasser Ghassan, Maresca Marc, Pochat-Bohatier Céline

机构信息

Institut Européen des Membranes, IEM UMR 5635, Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, France.

Platform for Research and Analysis in Environmental Sciences (PRASE), Lebanese University, Beirut, Lebanon.

出版信息

Antioxidants (Basel). 2021 Jan 7;10(1):68. doi: 10.3390/antiox10010068.

Abstract

The present study aims to investigate the properties of biopolymers extracted from a Lebanese onion non edible plant. The extraction was performed under mild conditions by varying the percentage of ultra-sound (US) treatment duration to a total extraction time of 30 min (0, 50, 100% US). The extracts were characterized using FTIR, SEC, GC-MS, TGA, and DSC analyses. The composition of the extracts was determined from the total carbohydrate content and protein content measurements. The thermal analyses indicate that all samples have high thermal stability. The antioxidant activities of the extracts were investigated, using β-carotene bleaching, scavenging activity of ABTS, metal chelating ability, and total antioxidant activity tests. The results indicate that the 50% US treatment leads to the best antioxidant activity. Biocompatibility of the extracts was evaluated using hemolysis and cytotoxicity assays. The results showed that 0 and 50% US samples are not toxic to human cells, in contrary to 100% US.

摘要

本研究旨在探究从黎巴嫩一种不可食用的洋葱植物中提取的生物聚合物的特性。通过改变超声(US)处理时间占总提取时间30分钟的百分比(0%、50%、100% US),在温和条件下进行提取。使用傅里叶变换红外光谱(FTIR)、尺寸排阻色谱(SEC)、气相色谱 - 质谱联用(GC-MS)、热重分析(TGA)和差示扫描量热法(DSC)分析对提取物进行表征。通过测量总碳水化合物含量和蛋白质含量来确定提取物的组成。热分析表明所有样品都具有高热稳定性。使用β - 胡萝卜素漂白法、ABTS清除活性、金属螯合能力和总抗氧化活性测试来研究提取物的抗氧化活性。结果表明,50% US处理导致最佳抗氧化活性。使用溶血和细胞毒性试验评估提取物的生物相容性。结果表明,0%和50% US处理的样品对人体细胞无毒,而100% US处理的样品则不然。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e63/7827233/ca1caecafb11/antioxidants-10-00068-g001.jpg

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