睑板腺囊肿的脂质

The lipids of chalazia.

作者信息

Nicolaides N, Flores A, Santos E C, Robin J B, Smith R E

机构信息

Department of Medicine/Dermatology, University of Southern California School of Medicine, Los Angeles 90033.

出版信息

Invest Ophthalmol Vis Sci. 1988 Mar;29(3):482-6.

PMID:3343104

Abstract

The tissue contents of chalazia taken from five individuals were extracted for lipids and analyzed by TLC and GLC. Lipids found were sterol esters 9%, wax esters 0%, triacyl glycerols 4%, free cholesterol 11%, free fatty acids 2%, unidentified nonpolar material 3%, ceramides 7%, cerebrosides 3%, phosphatidyl ethanolamine 12%, phosphatidyl choline 20%, sphingomyelin 4%, lysolecithin 4%, unidentified polar material 18.0%. GLC of the sterol esters gave peaks corresponding to the C14, C16, C18 and C20 normal fatty acid esters of cholesterol. The free fatty acids were also of the normal kind. Thus, the lipids of chalazia contents show little similarity to the lipids of the meibomian gland. The latter has a large component of sterol esters and wax esters. Also the fatty acids of all the meibomian gland ester lipids have significant amounts of iso- and anteisobranching in contrast to the chalazia lipids. Chalazia lipids more closely resemble the lipids of membranes of phagocytic cells with a large cholesterol content.

摘要

从五名个体取出的睑板腺囊肿组织内容物进行脂质提取，并通过薄层层析（TLC）和气相色谱法（GLC）进行分析。所发现的脂质有：甾醇酯9%、蜡酯0%、三酰甘油4%、游离胆固醇11%、游离脂肪酸2%、未鉴定的非极性物质3%、神经酰胺7%、脑苷脂3%、磷脂酰乙醇胺12%、磷脂酰胆碱20%、鞘磷脂4%、溶血卵磷脂4%、未鉴定的极性物质18.0%。甾醇酯的气相色谱分析给出了与胆固醇的C14、C16、C18和C20正常脂肪酸酯相对应的峰。游离脂肪酸也是正常类型的。因此，睑板腺囊肿内容物的脂质与睑板腺的脂质几乎没有相似之处。睑板腺含有大量的甾醇酯和蜡酯。而且，与睑板腺囊肿脂质相比，所有睑板腺酯脂质的脂肪酸都有大量的异支链和反异支链。睑板腺囊肿脂质更类似于具有高胆固醇含量的吞噬细胞膜的脂质。