Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, 10617, Taiwan.
Department of Dermatology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, 10002, Taiwan.
Sci Rep. 2021 Jan 11;11(1):329. doi: 10.1038/s41598-020-77889-z.
Imaging depth and quality of optical microscopy can be enhanced by optical clearing. Here we investigate the optical clearing of the ex vivo human skin by 50% glycerol topical application, which is allowed for cosmetic usage. Harmonic generation microscopy, by combining second and third harmonic generation (THG) modalities, was utilized to examine the clearing effect. The THG image intensity is sensitive to the improved optical homogeneity after optical clearing, and the second harmonic generation (SHG) image intensity in the dermis could serve as a beacon to confirm the reduction of the scattering in the epidermis layer. As a result, our study supports the OC effect through 50% glycerol topical application. Our study further indicates the critical role of stratum corneum shrinkage for the observed SHG and THG signal recovery.
光学透明化可以增强光学显微镜的成像深度和质量。在这里，我们通过 50%甘油的局部应用来研究离体人体皮肤的光学透明化，这种应用被允许用于化妆品。谐波产生显微镜通过结合二次和三次谐波产生（THG）模式来检查透明化效果。THG 图像强度对光学透明化后改善的光学均一性敏感，而真皮中的二次谐波产生（SHG）图像强度可以作为确认表皮层散射减少的标志。因此，我们的研究通过 50%甘油的局部应用支持 OC 效应。我们的研究进一步表明，角质层收缩对于观察到的 SHG 和 THG 信号恢复起着关键作用。
