Chang G, Absolom D R, Strong A B, Stubley G D, Zingg W
Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
J Biomed Mater Res. 1988 Jan;22(1):13-29. doi: 10.1002/jbm.820220104.
Using a flow cell design which ensures fully developed laminar flow, the influence of various hydrodynamic and physical factors in determining the extent of erythrocyte adhesion to various polymer surfaces has been examined. Specifically we have investigated the effect of exposure time, flow rate, erythrocyte concentration, and substrate surface tension on the extent of erythrocyte adhesion. The results indicate: (1) the extent of erythrocyte adhesion is most extensive on the more hydrophobic surfaces; (2) the rate of adhesion is higher on the more hydrophobic surfaces; (3) saturation coverage occurs after 7-10 min of exposure to the erythrocyte suspension for all substrates examined. No "lag-time" in the onset of adhesion was observed; (4) The level of saturation depends on the bulk erythrocyte concentration, increasing with increasing cell concentration; (5) the extent of adhesion decreases with an increase in flow rate; and (6) substrate surface defects such as roughness have a major effect on the pattern of erythrocyte adhesion.
采用能确保实现充分发展层流的流动池设计，研究了各种流体动力学和物理因素在确定红细胞与各种聚合物表面黏附程度方面的影响。具体而言，我们研究了暴露时间、流速、红细胞浓度和底物表面张力对红细胞黏附程度的影响。结果表明：（1）在疏水性更强的表面上，红细胞黏附程度最为广泛；（2）在疏水性更强的表面上，黏附速率更高；（3）对于所有检测的底物，暴露于红细胞悬液7 - 10分钟后会出现饱和覆盖。未观察到黏附开始时的“滞后时间”；（4）饱和水平取决于红细胞总体浓度，随细胞浓度增加而升高；（5）黏附程度随流速增加而降低；（6）诸如粗糙度等底物表面缺陷对红细胞黏附模式有重大影响。
