Absolom D R, Hawthorn L A, Chang G
Imaging Science Associates, Toronto, Ontario, Canada.
J Biomed Mater Res. 1988 Apr;22(4):271-85. doi: 10.1002/jbm.820220403.
The role that substrate surface properties play in influencing the extent of endothelialization of polymer surfaces has been investigated. For a wide range of polymer surfaces, the degree of endothelialization for both porcine and bovine endothelial cells is directly related to polymer surface tension: increased endothelialization occurring with increasing substrate surface tension. As a result of adsorption of the proteins in the culture media, the surface properties of the polymers are altered considerably. The protein-coated polymers were characterized by means of liquid-liquid contact angle measurements under non-denaturing conditions. A striking correlation is observed between the degree of endothelialization and the measured dextran contact angle. The degree of endothelial cell spreading is not related to polymer surface tension. Cell morphology and extracellular matrix production, however, are influenced by substrate surface properties.
已经研究了底物表面性质在影响聚合物表面内皮化程度方面所起的作用。对于多种聚合物表面，猪和牛内皮细胞的内皮化程度与聚合物表面张力直接相关：随着底物表面张力的增加，内皮化程度也增加。由于培养基中蛋白质的吸附，聚合物的表面性质发生了相当大的变化。通过在非变性条件下进行液-液接触角测量对蛋白质包被的聚合物进行了表征。在内皮化程度与测得的葡聚糖接触角之间观察到显著的相关性。内皮细胞的铺展程度与聚合物表面张力无关。然而，细胞形态和细胞外基质的产生受底物表面性质的影响。
