Wang Shu, Gu Miao, Luan Cheng-Cheng, Wang Yu, Gu Xiaosong, He Jiang-Hong
Key Laboratory for Neuroregeneration of Jiangsu Province and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China.
Department of Basic Medicine, Chengde Medical College, Chengde, Hebei Province, China.
Neural Regen Res. 2021 Aug;16(8):1606-1612. doi: 10.4103/1673-5374.303041.
In a previous study, we used natural butterfly wings as a cell growth matrix for tissue engineering materials and found that the surface of different butterfly wings had different ultramicrostructures, which can affect the qualitative growth of cells and regulate cell growth, metabolism, and gene expression. However, the biocompatibility and biosafety of butterfly wings must be studied. In this study, we found that Sprague-Dawley rat dorsal root ganglion neurons could grow along the structural stripes of butterfly wings, and Schwann cells could normally attach to and proliferate on different species of butterfly wings. The biocompatibility and biosafety of butterfly wings were further examined through subcutaneous implantation in Sprague-Dawley rats, intraperitoneal injection in Institute of Cancer Research mice, intradermal injection in rabbits, and external application to guinea pigs. Our results showed that butterfly wings did not induce toxicity, and all examined animals exhibited normal behaviors and no symptoms, such as erythema or edema. These findings suggested that butterfly wings possess excellent biocompatibility and biosafety and can be used as a type of tissue engineering material. This study was approved by the Experimental Animal Ethics Committee of Jiangsu Province of China (approval No. 20190303-18) on March 3, 2019.
在先前的一项研究中，我们将天然蝴蝶翅膀用作组织工程材料的细胞生长基质，发现不同蝴蝶翅膀的表面具有不同的超微结构，这会影响细胞的定性生长并调节细胞生长、代谢和基因表达。然而，蝴蝶翅膀的生物相容性和生物安全性必须进行研究。在本研究中，我们发现斯普拉格-道利大鼠背根神经节神经元能够沿着蝴蝶翅膀的结构条纹生长，雪旺细胞能够正常附着在不同种类的蝴蝶翅膀上并增殖。通过在斯普拉格-道利大鼠皮下植入、在癌症研究所小鼠腹腔注射、在兔子皮内注射以及在豚鼠体表涂抹，对蝴蝶翅膀的生物相容性和生物安全性进行了进一步检测。我们的结果表明，蝴蝶翅膀不会诱导毒性，所有受试动物行为正常且无红斑或水肿等症状。这些发现表明蝴蝶翅膀具有优异的生物相容性和生物安全性，可作为一种组织工程材料使用。本研究于2019年3月3日获得中国江苏省实验动物伦理委员会批准（批准号：20190303-18）。
