利用层流微尺度涡流从母体外周血中富集循环滋养层细胞。
Enrichment of circulating trophoblasts from maternal blood using laminar microscale vortices.
机构信息
Laboratory of Pharmaceutical Biotechnology, Ghent University, Gent, Belgium.
Department of Life Science Technologies, IMEC, Leuven, Belgium.
出版信息
Prenat Diagn. 2021 Aug;41(9):1171-1178. doi: 10.1002/pd.5901. Epub 2021 Feb 1.
OBJECTIVE
Enrichment of circulating trophoblasts (CTs) from maternal blood at week 11-13 of gestation, using laminar microscale vortices, and evaluation of the performance of the VTX-1 Liquid Biopsy System in terms of CT recovery and purity.
METHOD
Eight mililiter of blood was collected from 15 pregnant women and processed with the VTX-1 Liquid Biopsy System. Y-chromosome specific quantitative PCR was performed to estimate the number of enriched male CTs. To evaluate the VTX-1 performance, the target cell recovery was characterized by spiking experiments with a trophoblast cell line. Furthermore, the total quantity of DNA after enrichment was used to calculate the number of retained maternal cells.
RESULTS
Successful recovery of male CTs was established in 7 out of 10 first trimester samples from pregnant women carrying a male fetus. The number of CTs, recovered from 8 ml of blood, was estimated between two and six. Spiking experiments resulted in a CT recovery of ±35 % with ±1524 retained maternal blood cells.
CONCLUSION
CTs can be enriched from maternal blood with high purity, using laminar microscale vortices, starting from 8 ml of blood.
目的
利用层流微尺度涡旋从孕 11-13 周母血中富集循环滋养层细胞（CTs），并评估 VTX-1 液体活检系统在 CT 回收和纯度方面的性能。
方法
从 15 名孕妇采集 8 毫升血液，并通过 VTX-1 液体活检系统进行处理。采用 Y 染色体特异性定量 PCR 估计富集的男性 CT 数量。为了评估 VTX-1 的性能，通过用滋养层细胞系进行掺入实验来表征靶细胞的回收情况。此外，富集后总 DNA 量用于计算保留的母体细胞数量。
结果
在 10 名怀有男性胎儿的孕妇的 7 名孕早期样本中成功回收了男性 CTs。从 8 毫升血液中回收的 CT 数量估计为 2 至 6 个。掺入实验导致 CT 回收率为±35%，同时保留了±1524 个母体血液细胞。
结论
使用层流微尺度涡旋，可从 8 毫升血液中以高纯度富集 CTs。
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