Laboratory of Pharmaceutical Biotechnology, Ghent University, Gent, Belgium.
Department of Life Science Technologies, IMEC, Leuven, Belgium.
Prenat Diagn. 2021 Aug;41(9):1171-1178. doi: 10.1002/pd.5901. Epub 2021 Feb 1.
Enrichment of circulating trophoblasts (CTs) from maternal blood at week 11-13 of gestation, using laminar microscale vortices, and evaluation of the performance of the VTX-1 Liquid Biopsy System in terms of CT recovery and purity.
Eight mililiter of blood was collected from 15 pregnant women and processed with the VTX-1 Liquid Biopsy System. Y-chromosome specific quantitative PCR was performed to estimate the number of enriched male CTs. To evaluate the VTX-1 performance, the target cell recovery was characterized by spiking experiments with a trophoblast cell line. Furthermore, the total quantity of DNA after enrichment was used to calculate the number of retained maternal cells.
Successful recovery of male CTs was established in 7 out of 10 first trimester samples from pregnant women carrying a male fetus. The number of CTs, recovered from 8 ml of blood, was estimated between two and six. Spiking experiments resulted in a CT recovery of ±35 % with ±1524 retained maternal blood cells.
CTs can be enriched from maternal blood with high purity, using laminar microscale vortices, starting from 8 ml of blood.
利用层流微尺度涡旋从孕 11-13 周母血中富集循环滋养层细胞（CTs），并评估 VTX-1 液体活检系统在 CT 回收和纯度方面的性能。
从 15 名孕妇采集 8 毫升血液，并通过 VTX-1 液体活检系统进行处理。采用 Y 染色体特异性定量 PCR 估计富集的男性 CT 数量。为了评估 VTX-1 的性能，通过用滋养层细胞系进行掺入实验来表征靶细胞的回收情况。此外，富集后总 DNA 量用于计算保留的母体细胞数量。
在 10 名怀有男性胎儿的孕妇的 7 名孕早期样本中成功回收了男性 CTs。从 8 毫升血液中回收的 CT 数量估计为 2 至 6 个。掺入实验导致 CT 回收率为±35%，同时保留了±1524 个母体血液细胞。
使用层流微尺度涡旋，可从 8 毫升血液中以高纯度富集 CTs。
