Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Brazil.
Programa de Pós-Graduação em Ciências Ambientais, Universidade Federal de Goiás - Campus Samambaia, Goiânia, Brazil.
Chemosphere. 2021 May;271:129476. doi: 10.1016/j.chemosphere.2020.129476. Epub 2020 Dec 30.
The toxicity of zinc oxide (ZnO NPs) and polystyrene nanoplastics (PS NaPs) has been tested in different animal models; however, knowledge about their impact on mice remains incipient. The aim of the current study is to evaluate the effects of these nanomaterials on Swiss mice after their individual exposure to a binary combination of them. The goal was to investigate whether short exposure (three days) to an environmentally relevant dose (14.6 ng/kg, i.p.) of these pollutants would have neurotoxic, biochemical and genotoxic effects on the modelss. Data in the current study have shown that the individual exposure of these animals has led to cognitive impairment based on the object recognition test, although the exposure experiment did not cause locomotor and anxiogenic or anxiolitic-like behavioral changes in them. This outcome was associated with increased nitric oxide levels, thiobarbituric acid reactive species, reduction in acetylcholinesterase activity and with the accumulation of nanomaterials in their brains. Results recorded for the assessed parameters did not differ between the control group and the groups exposed to the binary combination of pollutants. However, both the individual and the combined exposures caused erythrocyte DNA damages associated with hypercholesterolemic and hypertriglyceridemic conditions due to the presence of nanomaterials. Based on the results, the toxicological potential of ZnO NPs and PS NaPs in the models was confirmed and it encouraged further in-depth investigations about factors explaining the lack of additive or synergistic effect caused by the combined exposure to the assessed pollutants.
氧化锌纳米粒子 (ZnO NPs) 和聚苯乙烯纳米塑料 (PS NaPs) 的毒性已在不同的动物模型中进行了测试；然而，关于它们对老鼠影响的知识仍处于初始阶段。本研究的目的是评估这些纳米材料在单独暴露于它们的二元混合物后对瑞士小鼠的影响。目的是研究短期暴露（三天）于环境相关剂量（14.6 ng/kg，腹腔内注射）的这些污染物是否会对模型产生神经毒性、生化和遗传毒性作用。目前研究的数据表明，这些动物的单独暴露导致了基于物体识别测试的认知障碍，尽管暴露实验并没有导致它们的运动和焦虑或抗焦虑样行为发生变化。这种结果与一氧化氮水平的升高、硫代巴比妥酸反应性物质的增加、乙酰胆碱酯酶活性的降低以及纳米材料在大脑中的积累有关。在评估的参数中，与对照组相比，暴露于二元混合物中的污染物的两组之间没有记录到差异。然而，无论是单独暴露还是联合暴露都导致了红细胞 DNA 损伤，这与纳米材料的存在有关，导致高胆固醇血症和高三酰甘油血症。基于这些结果，证实了 ZnO NPs 和 PS NaPs 在模型中的毒理学潜力，并鼓励进一步深入研究解释二元混合物暴露引起的缺乏相加或协同作用的因素。
