Estrela Fernanda Neves, Batista Guimarães Abraão Tiago, Silva Fabiano Guimarães, Marinho da Luz Thiarlen, Silva Abner Marcelino, Pereira Paulo Sergio, Malafaia Guilherme
Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Brazil.
Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, Urutaí, Brazil.
J Hazard Mater. 2021 Feb 5;403:123879. doi: 10.1016/j.jhazmat.2020.123879. Epub 2020 Sep 7.
The toxicity of polystyrene nanoparticles (PS NPs) and ZnO nanoparticles (ZnO NPs), in combination is poorly known. Thus, the aim of the current study was to evaluate the effects of PS NPs (760 μg/L) on Ctenopharyngodon idella exposed to it, both in separate and in combination with ZnO NPs (760 μg/L), based on behavioral, biochemical and genotoxic biomarkers. Current data have indicated that PS NPs, for a short exposure period (3 days), both in separate and in combination with nanoparticles, have affected animals' response to the mirror test. On the other hand, all treatments have equally induced C. idella inactivity towards alarm substances and DNA damage. There was increased oxidative stress, mainly in groups exposed to PS NPs (in combination, or not, with nanoparticles); although increased, the evaluated antioxidant levels did not appear to be enough to inhibit the effects of treatment-induced production of free radicals. Together, these results are likely co-responsible for the observed changes. The current study did not observe antagonistic, synergistic or additive effect on animals exposed to the combination between PS NPs and ZnO NPs; however, this outcome should not discourage the performance of similar studies focused on assessing the (eco)toxicity of pollutant mixtures comprising nanomaterials.
聚苯乙烯纳米颗粒（PS NPs）和氧化锌纳米颗粒（ZnO NPs）的联合毒性鲜为人知。因此，本研究的目的是基于行为、生化和遗传毒性生物标志物，评估PS NPs（760 μg/L）单独以及与ZnO NPs（760 μg/L）联合作用于草鱼时的影响。目前的数据表明，在短暴露期（3天）内，PS NPs单独以及与纳米颗粒联合作用时，均影响了动物对镜像测试的反应。另一方面，所有处理均同样诱导了草鱼对警报物质的无反应性和DNA损伤。氧化应激增加，主要出现在暴露于PS NPs的组中（无论是否与纳米颗粒联合）；尽管抗氧化水平有所增加，但似乎不足以抑制处理诱导的自由基产生的影响。这些结果共同可能是观察到的变化的原因。本研究未观察到PS NPs和ZnO NPs联合作用于动物时的拮抗、协同或相加效应；然而，这一结果不应阻碍开展类似研究以评估包含纳米材料的污染物混合物的（生态）毒性。
