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Microbial analysis for the ammonium removal from landfill leachate in an aerobic granular sludge sequencing batch reactor.
机构信息
Key Laboratory of Environmental Protection in Water Transport Engineering Ministry of Communications, Tianjin Research Institute of Water Transport Engineering, Tianjin 300456, China; School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.
Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.
出版信息
Bioresour Technol. 2021 Mar;324:124639. doi: 10.1016/j.biortech.2020.124639. Epub 2021 Jan 5.
In this study, a laboratory-scale sequencing batch reactor (SBR) equipped with aerobic granular sludge (AGS) technology was continuously operated for 220 days to remove ammonium from an existing landfill leachate. The ammonium removal was characterized by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology. This method helped to analyze the long-term community structural stability of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and denitrifying bacteria (DB) throughout the experiment. Simultaneously, 16S rRNA gene cloning and sequencing analysis identified the dominant species of different microbial species. Experimental results confirmed that ammonium removal was inhibited at the high nitrogen loading rate (NLR) stage while the low NLR stage achieved satisfactory ammonium removal. Moreover, the findings demonstrated that functionally stable wastewater treatment bioreactors facilitated the occurrence of stable microbial community structures.
在这项研究中，采用配备好氧颗粒污泥（AGS）技术的实验室规模序批式反应器（SBR）连续运行 220 天，以去除现有垃圾渗滤液中的铵。聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）技术用于对铵的去除进行了特征描述。该方法有助于分析整个实验过程中氨氧化细菌（AOB）、亚硝酸盐氧化细菌（NOB）和反硝化细菌（DB）的长期群落结构稳定性。同时，16S rRNA 基因克隆和测序分析确定了不同微生物物种的优势种。实验结果证实，在高氮负荷率（NLR）阶段，铵的去除受到抑制，而在低 NLR 阶段，铵的去除则令人满意。此外，研究结果表明，功能稳定的废水处理生物反应器有利于稳定的微生物群落结构的发生。
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