Sussman I P, MacGregor L C, Masters B R, Matschinsky F M
University of Pennsylvania, Department of Biochemistry and Biophysics, Philadelphia 19104.
J Histochem Cytochem. 1988 Mar;36(3):237-44. doi: 10.1177/36.3.3343507.
Carbon furnace atomic absorption spectrometry was used to measure the Na and K content of freeze-dried microscopic tissue samples. This method was sufficiently sensitive to measure pmol amounts of Na and K from tissue weighing 10-60 ng. Within the spatial resolution of the microdissection procedure, ion diffusion that might occur during cryosectioning, freeze-drying, and dissection of the tissue did not seem to be a problem. Data obtained with this methodology were in agreement with previously reported values of the Na and K content of various tissues, thus supporting the usefulness of this quantitative histochemical technique.
采用碳炉原子吸收光谱法测定冻干显微组织样本中的钠和钾含量。该方法灵敏度足够高，能够测量重10 - 60 ng组织中皮摩尔量的钠和钾。在显微切割程序的空间分辨率范围内，组织在冷冻切片、冻干和切割过程中可能发生的离子扩散似乎不是问题。用该方法获得的数据与先前报道的各种组织的钠和钾含量值一致，从而支持了这种定量组织化学技术的实用性。
