Choi Goro, Jeong Gu Min, Oh Myung Seok, Joo Munkyu, Im Sung Gap, Jeong Ki Jun, Lee Eunjung
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Daejeon 34141, Republic of Korea.
ACS Biomater Sci Eng. 2018 Jul 9;4(7):2614-2622. doi: 10.1021/acsbiomaterials.8b00241. Epub 2018 Jun 15.
Fabrication of new antibacterial surfaces has become a primary strategy for preventing device-associated infections (DAIs). Although considerable progress has recently been made in reducing DAIs, current antibacterial coating methods are technically complex and do not allow selective bacterial killing. Here, we propose novel anti-infective surfaces made of a cross-linked ionic polymer film that achieve selective bacteria killing while simultaneously favoring the survival of mammalian cells. A one-step polymerization process known as initiated chemical vapor deposition was used to generate a cross-linked ionic polymer film from 4-vinylbenzyl chloride and 2-(dimethylamino) ethyl methacrylate monomers in the vapor phase. In particular, the deposition process produced a polymer network with quaternary ammonium cross-linking sites, which provided the surface with an ionic moiety with an excellent antibacterial contact-killing property. This method confers substrate compatibility, which enables various materials to be coated with ionic polymer films for use in medical implants. Moreover, the ionic polymer-deposited surfaces supported the healthy growth of mammalian cells while selectively inhibiting bacterial growth in coculture models without any detectable cytotoxicity. Thus, the cross-linked ionic polymer-based antibacterial surface developed in this study can serve as an ideal platform for biomedical applications that require a highly sterile environment.
制造新型抗菌表面已成为预防与设备相关感染（DAIs）的主要策略。尽管最近在减少DAIs方面取得了相当大的进展，但目前的抗菌涂层方法技术复杂，且无法实现选择性细菌杀灭。在此，我们提出了一种由交联离子聚合物薄膜制成的新型抗感染表面，该表面能够实现选择性细菌杀灭，同时有利于哺乳动物细胞的存活。一种称为引发化学气相沉积的一步聚合工艺被用于从4-乙烯基苄基氯和甲基丙烯酸2-（二甲氨基）乙酯单体在气相中生成交联离子聚合物薄膜。特别地，沉积过程产生了具有季铵交联位点的聚合物网络，为表面提供了具有优异抗菌接触杀灭性能的离子部分。这种方法具有底物兼容性，能够使各种材料涂覆离子聚合物薄膜以用于医疗植入物。此外，在共培养模型中，离子聚合物沉积表面支持哺乳动物细胞的健康生长，同时选择性抑制细菌生长，且没有任何可检测到的细胞毒性。因此，本研究中开发的基于交联离子聚合物的抗菌表面可作为需要高度无菌环境的生物医学应用的理想平台。
