Frieler Madison, Pho Christine, Lee Bong Han, Dobrovolny Hana, Akkaraju Giridhar R, Naumov Anton V
Department of Biology, Texas Christian University, Fort Worth, TX 76129, USA.
Department of Physics and Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.
Nanomaterials (Basel). 2021 Jan 8;11(1):140. doi: 10.3390/nano11010140.
With 18 million new cases diagnosed each year worldwide, cancer strongly impacts both science and society. Current models of cancer cell growth and therapeutic efficacy in vitro are time-dependent and often do not consider the value (the maximum reduction in the growth rate), leading to inconsistencies in the obtained (concentration of the drug at half maximum effect). In this work, we introduce a new dual experimental/modeling approach to model HeLa and MCF-7 cancer cell growth and assess the efficacy of doxorubicin chemotherapeutics, whether alone or delivered by novel nitrogen-doped graphene quantum dots (N-GQDs). These biocompatible/biodegradable nanoparticles were used for the first time in this work for the delivery and fluorescence tracking of doxorubicin, ultimately decreasing its by over 1.5 and allowing for the use of up to 10 times lower doses of the drug to achieve the same therapeutic effect. Based on the experimental in vitro studies with nanomaterial-delivered chemotherapy, we also developed a method of cancer cell growth modeling that (1) includes an value, which is often not characterized, and (2), most importantly, is measurement time-independent. This will allow for the more consistent assessment of the efficiency of anti-cancer drugs and nanomaterial-delivered formulations, as well as efficacy improvements of nanomaterial delivery.
全球每年有1800万新确诊癌症病例，癌症对科学和社会都有重大影响。当前体外癌细胞生长和治疗效果模型依赖时间，且常常不考虑价值（生长速率的最大降低值），导致所获得的半数效应浓度不一致。在这项工作中，我们引入了一种新的双实验/建模方法来模拟HeLa和MCF - 7癌细胞生长，并评估阿霉素化疗药物的疗效，无论其单独使用还是通过新型氮掺杂石墨烯量子点（N - GQDs）递送。这些生物相容性/可生物降解的纳米颗粒在本研究中首次用于阿霉素的递送和荧光追踪，最终将其半数效应浓度降低超过1.5倍，并允许使用低至十分之一剂量的药物来达到相同的治疗效果。基于纳米材料递送化疗的体外实验研究，我们还开发了一种癌细胞生长建模方法，该方法（1）纳入了一个通常未被表征的价值，（2）最重要的是，与测量时间无关。这将使抗癌药物和纳米材料递送制剂的效率评估更加一致，以及纳米材料递送的疗效得到提高。
