在成年斑马鱼模型中，长期暴露于乙醇会改变酒精性肝病中miR-155、miR-122和miR-217的表达。
Chronic exposure to ethanol alters the expression of miR-155, miR-122 and miR-217 in alcoholic liver disease in an adult zebrafish model.
作者信息
Pasqualotto Amanda, Ayres Raquel, Longo Larisse, Del Duca Lima Diego, Losch de Oliveira Diogo, Alvares-da-Silva Mário Reis, Reverbel da Silveira Themis, Uribe-Cruz Carolina
机构信息
Experimental Hepatology and Gastroenterology Laboratory, Center for Experimental Research, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
Graduate Program in Gastroenterology and Hepatology, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Biomarkers. 2021 Mar;26(2):146-151. doi: 10.1080/1354750X.2021.1874051. Epub 2021 Jan 17.
AIM
The aim of this study was to evaluate the hepatic and circulating expression of miR-155, miR-122 and miR-217 in a model of chronic exposure to ethanol in adult zebrafish.
METHODS
Wild-type adult zebrafish were divided into two groups ( = 281): an EG (exposed to 0.5% v/v Ethanol in aquarium water) and a CG (without ethanol). After 28 days the animals were euthanized, followed by histopathological analysis, quantification of lipids, triglycerides and inflammatory cytokines in liver tissue. miR-155, miR-122 and miR-217 gene expression was quantified in liver tissue and serum.
RESULTS
We observed hepatic lesions and increased accumulation of hepatic lipids in the EG. The expression of was higher in the EG, but there were no differences in and between groups. In the liver, expression of miR-122 and miR-155 was higher in the EG. The circulating expression of miR-155 and miR-217 was significantly higher in the EG.
CONCLUSION
Chronic exposure to ethanol in zebrafish leads to altered hepatic and circulating expression of miR-155, miR-122 and miR-217. This confirms its potential as a biomarker and therapeutic target.
目的
本研究旨在评估成年斑马鱼慢性乙醇暴露模型中miR-155、miR-122和miR-217在肝脏及循环系统中的表达情况。
方法
将野生型成年斑马鱼分为两组（每组n = 281）：乙醇暴露组（EG，暴露于水族箱水中0.5% v/v乙醇）和对照组（CG，未接触乙醇）。28天后对动物实施安乐死，随后进行组织病理学分析、肝脏组织中脂质、甘油三酯及炎性细胞因子的定量检测。对肝脏组织和血清中的miR-155、miR-122和miR-217基因表达进行定量分析。
结果
我们观察到乙醇暴露组存在肝脏损伤及肝脏脂质蓄积增加的情况。乙醇暴露组中[此处原文缺失具体物质]的表达较高，但两组间[此处原文缺失具体物质]和[此处原文缺失具体物质]无差异。在肝脏中，乙醇暴露组中miR-122和miR-155的表达较高。乙醇暴露组中miR-155和miR-217的循环表达显著升高。
结论
斑马鱼慢性乙醇暴露导致miR-155、miR-122和miR-217在肝脏及循环系统中的表达发生改变。这证实了其作为生物标志物和治疗靶点的潜力。
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