College of Resource and Environmental Engineering, Wuhan University of Science and Technology , Wuhan, China.
Hubei Key Laboratory for Efficient Utilization and Agglomeration of Metallurgic Mineral Resources, Wuhan University of Science and Technology , Wuhan, Hubei, China.
Biotechnol Genet Eng Rev. 2020 Oct;36(2):81-131. doi: 10.1080/02648725.2020.1864187. Epub 2021 Jan 12.
Laccases have enormous potential as promising 'green' biocatalysts in environmental applications including wastewater treatment and polluted soil bioremediation. The catalytic oxidation reaction they perform uses only molecular oxygen without other cofactors, and the only product after the reaction is water. The immobilization of laccase offers several improvements such as protected activity and enhanced stability over free laccase. In addition, the reusability of immobilized laccase is adistinct advantage for future applications. This review covers the sources of and progress in laccase research, and discusses the different methodologies of laccase immobilization that have emerged in the recent 5-10 years, as well as its applications to environmental fields, and evaluates these emerging technologies. (2,4,6-TCP): 2,4,6-trichlorophenol; (2,4-DCP): 2,4-dichlorophenol; (ABTS), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid); (ACE), acetaminophen; (BC-AS), almond shell; (BC-PM), pig manure; (BC-PW), pine wood; (BPA), bisphenol A; (BPA), bisphenol A; (BPF), bisphenol F; (BPS), bisphenol S; (C), fullerene; (Ca-AIL), calcium-alginate immobilized laccase; (CBZ), carbamazepine; (CETY), cetirizine; (CHT-PGMA-PEI-Cu (II) NPs), Cu (II)-chelated chitosan nanoparticles; (CLEAs), cross-linked enzyme aggregates; (CMMC), carbon-based mesoporous magnetic composites; (COD), chemical oxygen demand; (CPH), ciprofloxacin hydrochloride; (CS), chitosan; (CTC), chlortetracycline; (Cu-AIL), copper-alginate immobilized laccase; (DBR K-4BL), Drimarene brilliant red K-4BL; (DCF), diclofenac; (E1),estrone; (E2), 17 β-estradiol; (EDC), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; (EDCs), endocrine disrupting chemicals; (EE2), 17α-ethinylestradiol; (EFMs), electrospun fibrous membranes; (FL), free laccase; (fsMP), fumed silica microparticles; (GA-CBs), GLU-crosslinked chitosan beads; (GA-CBs), glutaraldehyde-crosslinked chitosan beads; (GA-Zr-MOF), graphene aerogel-zirconium-metal organic framework; (GLU), glutaraldehyde; (GO), graphene oxide; (HMCs), hollow mesoporous carbon spheres; (HPEI/PES), hyperbranched polyethyleneimine/polyether sulfone; (IC), indigo carmine; (IL), immobilized laccase; (k), catalytic constant; (K), Michealis constant; (M-CLEAs), Magnetic cross-linked enzyme aggregates; (MMSNPs-CPTS-IDA-Cu), Cu-chelated magnetic mesoporous silica nanoparticles; (MSS), magnetic mesoporous silica spheres; (MWNTs), multi-walled carbon nanotubes; (MWNTs), multi-walled carbon nanotubes; (NHS), N-hydroxy succinimide; (O-MWNTs), oxidized-MWNTs; (P(AAm-NIPA)), poly(acrylamide-N-isopropylacrylamide); (p(GMA)), poly(glycidyl methacrylate); (p(HEMA)), poly(hydroxyethyl methacrylate); (p(HEMA-g-GMA)-NH, poly(glycidyl methacrylate) brush grafted poly(hydroxyethyl methacrylate); (PA6/CHIT), polyamide 6/chitosan; (PAC), powdered active carbon; (PAHs), polycyclic aromatic hydrocarbons; (PAM-CTS), chitosan grafted polyacrylamide hydrogel; (PAN/MMT/GO), polyacrylonitrile/montmorillonite/graphene oxide; (PAN/PVdF), polyacrylonitrile/polyvinylidene fluoride; (PEG), poly ethylene glycol; (PEI), Poly(ethyleneimine); (poly(4-VP)), poly(4-vinyl pyridine); (poly(GMA-MAA)), poly(glycidyl methacrylate-methacrylic acid); (PVA), polyvinyl alcohol; (RBBR), Remazol Brilliant Blue R; (SDE), simulated dye effluent; (semi-IPNs), semi-interpenetrating polymer networks; (TC), tetracycline; (TCH), tetracycline hydrochloride; (TCS), triclosan; (Vmax), maximum activity; (Zr-MOF, MMU), micro-mesoporous Zr-metal organic framework.
漆酶作为有前途的“绿色”生物催化剂，在环境应用中具有巨大的潜力，包括废水处理和污染土壤生物修复。它们进行的催化氧化反应仅使用分子氧，而不需要其他辅助因子，并且反应后的唯一产物是水。漆酶的固定化提供了一些改进，例如保护活性和提高游离漆酶的稳定性。此外，固定化漆酶的可重复使用性是未来应用的一个明显优势。 本文综述了漆酶研究的来源和进展，并讨论了最近 5-10 年来出现的不同漆酶固定化方法，以及其在环境领域的应用，并对这些新兴技术进行了评价。 (2,4,6-TCP)：2,4,6-三氯苯酚; (2,4-DCP)：2,4-二氯苯酚; (ABTS)，2,2-偶氮双 (3-乙基苯并噻唑啉-6-磺酸); (ACE)，对乙酰氨基酚; (BC-AS)，杏仁壳; (BC-PM)，猪粪; (BC-PW)，松木; (BPA)，双酚 A; (BPA)，双酚 A; (BPF)，双酚 F; (BPS)，双酚 S; (C)，富勒烯; (Ca-AIL)，海藻酸钙固定化漆酶; (CBZ)，卡马西平; (CETY)，西替利嗪; (CHT-PGMA-PEI-Cu(II) NPs)，Cu(II)螯合壳聚糖纳米颗粒; (CLEAs)，交联酶聚集体; (CMMC)，基于碳的中孔磁性复合材料; (COD)，化学需氧量; (CPH)，盐酸环丙沙星; (CS)，壳聚糖; (CTC)，金霉素; (Cu-AIL)，海藻酸铜固定化漆酶; (DBR K-4BL)，Drimarine 亮红 K-4BL; (DCF)，双氯芬酸; (E1)，雌酮; (E2)，17β-雌二醇; (EDC)，1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐; (EDCs)，内分泌干扰物; (EE2)，17α-乙炔雌二醇; (EFMs)，静电纺丝纤维膜; (FL)，游离漆酶; (fsMP)，气相二氧化硅微颗粒; (GA-CBs)，GLU 交联壳聚糖珠; (GA-CBs)，戊二醛交联壳聚糖珠; (GA-Zr-MOF)，石墨烯气凝胶-锆-金属有机骨架; (GLU)，戊二醛; (GO)，氧化石墨烯; (HMCs)，中空介孔碳球; (HPEI/PES)，超支化聚乙烯亚胺/聚醚砜; (IC)，靛蓝胭脂红; (IL)，固定化漆酶; (k)，催化常数; (K)，米氏常数; (M-CLEAs)，磁性交联酶聚集体; (MMSNPs-CPTS-IDA-Cu)，Cu 螯合磁性介孔硅纳米颗粒; (MSS)，磁性介孔硅球; (MWNTs)，多壁碳纳米管; (MWNTs)，多壁碳纳米管; (NHS)，N-羟基琥珀酰亚胺; (O-MWNTs)，氧化多壁碳纳米管; (P(AAm-NIPA))，聚丙烯酰胺-异丙基丙烯酰胺; (p(GMA))，聚 (甲基丙烯酸缩水甘油酯); (p(HEMA))，聚 (羟乙基甲基丙烯酸酯); (p(HEMA-g-GMA)-NH，聚 (甲基丙烯酸缩水甘油酯)刷接枝聚 (羟乙基甲基丙烯酸酯); (PA6/CHIT)，聚酰胺 6/壳聚糖; (PAC)，粉末活性炭; (PAHs)，多环芳烃; (PAM-CTS)，壳聚糖接枝聚丙烯酰胺水凝胶; (PAN/MMT/GO)，聚丙烯腈/蒙脱土/氧化石墨烯; (PAN/PVdF)，聚丙烯腈/聚偏二氟乙烯; (PEG)，聚乙二醇; (PEI)，聚乙烯亚胺; (poly(4-VP))，聚 (4-乙烯基吡啶); (poly(GMA-MAA))，聚 (甲基丙烯酸缩水甘油酯-甲基丙烯酸); (PVA)，聚乙烯醇; (RBBR)，丽春红 Brilliant Blue R; (SDE)，模拟染料废水; (半互穿网络)，半互穿聚合物网络; (TC)，四环素; (TCH)，盐酸四环素; (TCS)，三氯生; (Vmax)，最大活性; (Zr-MOF, MMU)，微孔-介孔 Zr 金属有机骨架。
