Center of Excellence for Innovation in Chemistry and Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand; Analytical Chemistry and Environment Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani, 94000, Thailand; Department of Chemistry/Biochemistry/Molecular Biology, Alex-Ekwueme Federal University, Ndufu-Alike-Ikwo, Abakaliki, Ebonyi State, Nigeria.
Center of Excellence for Innovation in Chemistry and Division of Physical Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Carbohydr Polym. 2021 Mar 1;255:117376. doi: 10.1016/j.carbpol.2020.117376. Epub 2020 Nov 5.
A sensitive detection strategy for ascorbic acid (AA), using sodium alginate reduced/stabilized gold nanoparticles (SA-AuNPs) as the optical probe, is reported. The SA-AuNPs were prepared by mixing gold salt and SA under stirring for 2 h at room temperature, without any further steps. The mixture was aged at 4 °C overnight, after which a faint-purple colloidal solution of SA-AuNPs was obtained. Characterization shows that the synthesis is incapable of reducing all Au to Au°, but rather to mixture of Au°/Au. The addition of AA to the SA-AuNPs probe reduced completely all Au to new AuNPs which were deposited on the pre-formed SA-AuNPs seed, leading to size increment and absorption spectra enhancement. The assay exhibited a good linearity between 12.5 and 150.0 μM AA and low limit of quantification of 11.2 μM. It was further used for AA quantitation in vitamin C injection and fruit juice with satisfactory accuracy and precision.
本文报道了一种灵敏的检测抗坏血酸（AA）的策略，使用海藻酸钠还原/稳定的金纳米粒子（SA-AuNPs）作为光学探针。SA-AuNPs 通过在室温下搅拌混合金盐和 SA 制备而成，无需进一步的步骤。将混合物在 4°C 下陈化过夜，得到淡紫色胶体的 SA-AuNPs 溶液。表征表明，该合成方法不能将所有的 Au 还原为 Au°，而是形成 Au°/Au 的混合物。向 SA-AuNPs 探针中加入 AA 可将所有的 Au 完全还原为新的 AuNPs，这些 AuNPs 沉积在预先形成的 SA-AuNPs 种子上，导致尺寸增加和吸收光谱增强。该分析方法在 12.5 至 150.0 μM AA 之间呈现良好的线性关系，定量下限为 11.2 μM。该方法进一步用于维生素 C 注射液和果汁中 AA 的定量，具有令人满意的准确性和精密度。
