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利用光镊提高原子力显微镜胶体探针的精度。

Increasing AFM colloidal probe accuracy by optical tweezers.

作者信息

Witko Tomasz, Baster Zbigniew, Rajfur Zenon, Sofińska Kamila, Barbasz Jakub

机构信息

M. Smoluchowski Institute of Physics, Jagiellonian University, Łojasiewicza 11, 30-348, Kraków, Poland.

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239, Kraków, Poland.

出版信息

Sci Rep. 2021 Jan 12;11(1):509. doi: 10.1038/s41598-020-79938-z.

DOI:10.1038/s41598-020-79938-z
PMID:33436725
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7804458/
Abstract

A precise determination of the cantilever spring constant is the critical point of all colloidal probe experiments. Existing methods are based on approximations considering only cantilever geometry and do not take into account properties of any object or substance attached to the cantilever. Neglecting the influence of the colloidal sphere on the cantilever characteristics introduces significant uncertainty in a spring constant determination and affects all further considerations. In this work we propose a new method of spring constant calibration for 'colloidal probe' type cantilevers based on the direct measurement of force constant. The Optical Tweezers based calibration method will help to increase the accuracy and repeatability of the AFM colloidal probe experiments.

摘要

精确测定悬臂梁的弹簧常数是所有胶体探针实验的关键点。现有方法基于仅考虑悬臂梁几何形状的近似值，并未考虑附着在悬臂梁上的任何物体或物质的特性。忽略胶体球对悬臂梁特性的影响会在弹簧常数测定中引入显著的不确定性，并影响所有后续考量。在这项工作中，我们基于力常数的直接测量，提出了一种针对“胶体探针”型悬臂梁的弹簧常数校准新方法。基于光镊的校准方法将有助于提高原子力显微镜胶体探针实验的准确性和可重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/e39fb00b8d33/41598_2020_79938_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/48206cfb701a/41598_2020_79938_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/b00a119ca569/41598_2020_79938_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/2c304ef9afb2/41598_2020_79938_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/bce18fc2d783/41598_2020_79938_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/e39fb00b8d33/41598_2020_79938_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/48206cfb701a/41598_2020_79938_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/b00a119ca569/41598_2020_79938_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/2c304ef9afb2/41598_2020_79938_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/bce18fc2d783/41598_2020_79938_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b687/7804458/e39fb00b8d33/41598_2020_79938_Fig5_HTML.jpg

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