Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1 Josui-honcho, Kodaira, Tokyo, 187-0022, Japan.
Functional Materials Department, Institute of Technology Solutions, R&D Division, Kewpie Corporation, Sengawa Kewport, 2-5-7 Sengawa-cho, Chofu, Tokyo, 182-0002, Japan.
Sci Rep. 2021 Jan 12;11(1):526. doi: 10.1038/s41598-020-80239-8.
Foot-and-mouth disease (FMD) is one of the most contagious diseases of cloven-hoofed animals. Disinfectants are used to inactivate FMD virus (FMDV) in Japan. Reports that heat-denatured lysozyme inactivates bacteria as well as viruses, such as norovirus and hepatitis A virus, led us to determine its effects on FMDV. We show here that heat-denatured lysozyme partially inhibited the infectivity of FMDV O/JPN/2010-1/14C but of FMDVs A/TAI/46-1/2015 and Asia1/Shamir (ISR/3/89). Further, heat-denatured lysozyme variably reduced RNA loads of FMDVs O/JPN/2010-1/14C, O/MOG/2/Ca/BU/2017, O/Taiwan/1997, Asia1/Shamir (ISR/3/89), Asia1/TUR/49/2011, SAT1/KEN/117/2009, SAT2/SAU/6/2000 and SAT3/ZIM/3/83 but could not those of O/JPN/2000, A/TAI/46-1/2015, A/IRQ/24/64, A/TAI/1/60 and C/PHI/7/84. These findings indicate that heat-denatured lysozyme may serve as a new disinfectant against FMDV.
口蹄疫（FMD）是偶蹄动物中最具传染性的疾病之一。在日本，消毒剂用于使口蹄疫病毒（FMDV）失活。有报道称，热变性溶菌酶可使细菌和病毒（如诺如病毒和甲型肝炎病毒）失活，这促使我们研究其对口蹄疫病毒的作用。我们在此表明，热变性溶菌酶部分抑制了口蹄疫病毒 O/JPN/2010-1/14C 的感染性，但不抑制口蹄疫病毒 A/TAI/46-1/2015 和 Asia1/Shamir（ISR/3/89）。此外，热变性溶菌酶可降低口蹄疫病毒 O/JPN/2010-1/14C、O/MOG/2/Ca/BU/2017、O/Taiwan/1997、Asia1/Shamir（ISR/3/89）、Asia1/TUR/49/2011、SAT1/KEN/117/2009、SAT2/SAU/6/2000 和 SAT3/ZIM/3/83 的 RNA 载量，但不能降低 O/JPN/2000、A/TAI/46-1/2015、A/IRQ/24/64、A/TAI/1/60 和 C/PHI/7/84 的 RNA 载量。这些发现表明，热变性溶菌酶可能成为一种新的抗口蹄疫病毒消毒剂。
