Department of Geriatrics, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Department of Radiology, Peking University Third Hospital, Beijing 100191, China.
Int J Med Sci. 2021 Jan 1;18(3):652-659. doi: 10.7150/ijms.54320. eCollection 2021.
Different anesthetics have distinct effects on the interstitial fluid (ISF) drainage in the extracellular space (ECS) of the superficial rat brain, while their effects on ISF drainage in the ECS of the deep rat brain still remain unknown. Herein, we attempt to investigate and compare the effects of propofol and isoflurane on ECS structure and ISF drainage in the caudate-putamen (CPu) and thalamus (Tha) of the deep rat brain. Adult Sprague-Dawley rats were anesthetized with propofol or isoflurane, respectively. Twenty-four anesthetized rats were randomly divided into the propofol-CPu, isoflurane-CPu, propofol-Tha, and isoflurane-Tha groups. Tracer-based magnetic resonance imaging (MRI) and fluorescent-labeled tracer assay were utilized to quantify ISF drainage in the deep brain. The half-life of ISF in the propofol-CPu and propofol-Tha groups was shorter than that in the isoflurane-CPu and isoflurane-Tha groups, respectively. The ECS volume fraction in the propofol-CPu and propofol-Tha groups was much higher than that in the isoflurane-CPu and isoflurane-Tha groups, respectively. However, the ECS tortuosity in the propofol-CPu and propofol-Tha groups was much smaller than that in isoflurane-CPu and isoflurane-Tha groups, respectively. Our results demonstrate that propofol rather than isoflurane accelerates the ISF drainage in the deep rat brain, which provides novel insights into the selective control of ISF drainage and guides selection of anesthetic agents in different clinical settings, and unravels the mechanism of how general anesthetics function.
不同的麻醉剂对浅层大鼠脑细胞外空间（ECS）间质液（ISF）的引流具有不同的影响，而它们对深层大鼠脑 ECS 中 ISF 引流的影响仍不清楚。在此，我们试图研究和比较异丙酚和异氟醚对深层大鼠脑尾状核（CPu）和丘脑（Tha）ECS 结构和 ISF 引流的影响。 成年 Sprague-Dawley 大鼠分别用异丙酚或异氟醚麻醉。24 只麻醉大鼠随机分为异丙酚-CPu、异氟醚-CPu、异丙酚-Tha 和异氟醚-Tha 组。示踪剂磁共振成像（MRI）和荧光标记示踪剂检测用于定量深部脑内 ISF 引流。 异丙酚-CPu 和异丙酚-Tha 组 ISF 的半衰期均短于异氟醚-CPu 和异氟醚-Tha 组。异丙酚-CPu 和异丙酚-Tha 组的 ECS 体积分数明显高于异氟醚-CPu 和异氟醚-Tha 组。然而，异丙酚-CPu 和异丙酚-Tha 组的 ECS 曲折度明显小于异氟醚-CPu 和异氟醚-Tha 组。 我们的结果表明，异丙酚而非异氟醚加速了深层大鼠脑 ISF 的引流，这为选择性控制 ISF 引流提供了新的见解，并指导了不同临床环境下麻醉剂的选择，揭示了全身麻醉剂作用的机制。
