Biochemistry Department, Faculty of Science, Ege University, Izmir, Turkey.
Center for Drug Research, Development and Pharmacokinetic Applications (ARGEFAR), Ege University, Izmir, Turkey.
Anticancer Agents Med Chem. 2021;21(18):2563-2571. doi: 10.2174/1871520621666210112123528.
Green synthesis, an alternative method for synthesizing nanoparticles, is cheaper, environmentally friendly, and does not show toxic effects. Doxorubicin is a chemotherapeutic agent used in lung cancer. Curcumin is a bioactive compound with properties, such as an anticancer obtained from Curcuma longa.
The objective of this study was to develop Doxorubicin and Curcumin loaded magnetic nanoparticles that could be synthesized by green tea leaves and to investigate cytotoxic effects against the A549-luc-C8, non-small cell lung cancer line.
Magnetic nanoparticles were synthesized with the green synthesis method. Furthermore, Doxorubicin and Curcumin were encapsulated into magnetic nanoparticles with the one-pot method and obtained magnetic nanoparticles characterized using FTIR, SEM/EDX, XRD, and UV-VIS spectrophotometric techniques. After that, The drug release test was performed by dialysis using pH 7.4 phosphate-buffered saline at 37 °C. MTT assay was performed to test the cytotoxicity effect in the A549-luc-C8 cell line.
FTIR analysis verified the magnetic structure and drug loading. SEM images of magnetic nanoparticle revealed that they had a size of about 50-60 nm in a mono-disperse manner. Drug release after 24 h was found to be 5.8% for doxorubicin and 3.4% for curcumin, showing controlled release.
Results showed that the prepared magnetic nanoparticles had a synergistic antitumor activity for A549-luc-C8.
绿色合成是一种合成纳米粒子的替代方法，它更便宜、环保，并且没有显示出毒性作用。阿霉素是一种用于肺癌的化疗药物。姜黄素是一种具有抗癌特性的生物活性化合物，可从姜黄中获得。
本研究旨在开发可通过绿茶叶片合成的载有阿霉素和姜黄素的磁性纳米粒子，并研究其对非小细胞肺癌 A549-luc-C8 细胞系的细胞毒性作用。
采用绿色合成法合成磁性纳米粒子。此外，通过一锅法将阿霉素和姜黄素包封到磁性纳米粒子中，并采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜/能谱（SEM/EDX）、X 射线衍射（XRD）和紫外可见分光光度法（UV-VIS）对所得磁性纳米粒子进行表征。然后，在 37℃下用 pH7.4 磷酸盐缓冲盐水进行透析法进行药物释放试验。采用 MTT 法测定阿霉素和姜黄素载药磁性纳米粒子对 A549-luc-C8 细胞系的细胞毒性作用。
FTIR 分析验证了磁性结构和药物负载。磁性纳米粒子的 SEM 图像显示，它们以单分散的方式具有约 50-60nm 的尺寸。在 24 小时后，阿霉素和姜黄素的药物释放量分别为 5.8%和 3.4%，表现出控释作用。
结果表明，所制备的磁性纳米粒子对 A549-luc-C8 具有协同抗肿瘤活性。
