Moacă Elena-Alina, Watz Claudia, Faur Alexandra-Corina, Lazăr Daniela, Socoliuc Vlad, Păcurariu Cornelia, Ianoș Robert, Rus Cristiana-Iulia, Minda Daliana, Barbu-Tudoran Lucian, Dehelean Cristina Adriana
Faculty of Pharmacy, "Victor Babes" University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania.
Research Centre for Pharmaco-Toxicological Evaluation, "Victor Babes" University of Medicine and Pharmacy, 2nd Eftimie Murgu Square, RO-300041 Timisoara, Romania.
Pharmaceutics. 2022 Dec 20;15(1):2. doi: 10.3390/pharmaceutics15010002.
The present study reports the successful synthesis of biocompatible magnetic iron oxide nanoparticles (MNPs) by an ecofriendly single step method, using two ethanolic extracts based on leaves of L. and L. The effect of both green raw materials as reducing and capping agents was taken into account for the development of MNPs, as well as the reaction synthesis temperature (25 °C and 80 °C). The biological effect of the MNPs obtained from L. ethanolic extract (Cs 25, Cs 80) was compared with that of the MNPs obtained from L. ethanolic extract (Ob 25, Ob 80), by using two morphologically different lung cancer cell lines (A549 and NCI-H460); the results showed that the higher cell viability impairment was manifested by A549 cells after exposure to MNPs obtained from L. ethanolic extract (Ob 25, Ob 80). Regarding the biosafety profile of the MNPs, it was shown that the EpiAirway models did not elicit important viability decrease or significant histopathological changes after treatment with none of the MNPs (Cs 25, Cs 80 and Ob 25, Ob 80), at concentrations up to 500 µg/mL.
本研究报告了一种通过环保单步方法成功合成生物相容性磁性氧化铁纳米颗粒（MNPs）的过程，该方法使用了基于L.和L.叶子的两种乙醇提取物。在MNPs的制备过程中，考虑了这两种绿色原料作为还原剂和封端剂的作用，以及反应合成温度（25°C和80°C）。通过使用两种形态不同的肺癌细胞系（A549和NCI-H460），比较了从L.乙醇提取物（Cs 25、Cs 80）获得的MNPs与从L.乙醇提取物（Ob 25、Ob 80）获得的MNPs的生物学效应；结果表明，A549细胞在暴露于从L.乙醇提取物（Ob 25、Ob 80）获得的MNPs后，表现出更高的细胞活力损伤。关于MNPs的生物安全性，结果表明，在浓度高达500μg/mL的情况下，用任何一种MNPs（Cs 25、Cs 80和Ob 25、Ob 80）处理后，EpiAirway模型均未引起重要的活力下降或显著的组织病理学变化。
