Unit of Subintensive Neonatal Care, Department of Pediatrics, Regina Margherita Children's Hospital, Città della Salute e della Scienza di Torino, Turin, Italy.
Unit of Neonatal Intensive Care, Department of Surgical Sciences, University of Cagliari, Cagliari, Italy.
Minerva Pediatr (Torino). 2024 Jun;76(3):404-413. doi: 10.23736/S2724-5276.20.06128-9. Epub 2021 Jan 13.
Lactobacillus reuteri DSM 17938 is the only probiotic recommended for treatment of colicky infants, but its mechanism of action is not clear. The study aim was to examine urinary metabolomic fingerprint of colicky breastfed infants before and after 1 month of orally administered Lactobacillus reuteri DSM 17938 or placebo.
This randomized, blinded, placebo-controlled clinical trial was carried out with a well-documented probiotic. Thirty-two infants were enrolled, 16 in the probiotic group and 16 in the placebo group. Urine samples were collected from each subject before starting supplementation and at the end of the study period. Metabolomic profiles were obtained using a gas chromatography/mass spectrometry instrument. Subsequently, to compare groups before and after probiotic supplementation, univariate and multivariate statistical analysis were performed.
In the L. reuteri treated group all metabolites for all class of nutrients (sugars, amino acids, carboxylic acids) resulted more abundant after the study period. The comparison with a control group (placebo treated), confirmed this effect on urines.
The metabolomic analysis of urine samples from infants treated with L. reuteri DSM 17938 allowed to detect some interesting features related to the effect of this treatment on urinary metabolome. To validate the results, a test on a larger cohort is required.
鼠李糖乳杆菌 DSM 17938 是唯一被推荐用于治疗绞痛婴儿的益生菌，但它的作用机制尚不清楚。本研究旨在观察口服鼠李糖乳杆菌 DSM 17938 或安慰剂治疗前后绞痛母乳喂养婴儿的尿代谢组指纹图谱。
这是一项随机、双盲、安慰剂对照的临床试验，使用了一种有充分文献记载的益生菌。共纳入 32 名婴儿，益生菌组 16 名，安慰剂组 16 名。在开始补充前和研究结束时收集每个受试者的尿液样本。使用气相色谱/质谱仪获得代谢组学图谱。随后，为了比较益生菌补充前后的组间差异，进行了单变量和多变量统计分析。
在接受鼠李糖乳杆菌治疗的组中，所有营养素类别（糖、氨基酸、羧酸）的代谢物在研究结束后都更加丰富。与对照组（安慰剂治疗）的比较证实了这对尿液的影响。
对接受鼠李糖乳杆菌 DSM 17938 治疗的婴儿尿液样本的代谢组学分析，能够检测到一些与这种治疗对尿液代谢组影响相关的有趣特征。为了验证结果，需要在更大的队列中进行测试。
