Department of Food Science & Technology, University of California-Davis, 1 Shields Avenue, Davis, California 95616, United States.
Institute for Global Nutrition and Department of Nutrition, University of California-Davis, 1 Shields Avenue, Davis, California 95616, United States.
J Agric Food Chem. 2021 Jan 27;69(3):1107-1114. doi: 10.1021/acs.jafc.0c06144. Epub 2021 Jan 13.
Saccharin and -resveratrol were incorporated into small quantity lipid-based nutritional supplements (SQ-LNS) to be evaluated as the markers of consumption for nutritional intervention studies. Forty-seven healthy women consumed a single supplement with either 8.6 mg of saccharin or 5 mg of -resveratrol, and urine was collected for 4 h. A rapid 11 min method employing multiple reaction monitoring and ultrahigh performance liquid chromatography coupled to a triple quadrupole mass spectrometer was developed to measure saccharin and resveratrol metabolites in urine simultaneously. The linear dynamic range of the method was from 3 to 1000 ng mL, with the correlation coefficient of 0.999 and limits of quantification from 15.28 to 53.03 ng mL. Sample preparation was simple dilution with an average recovery of 97.8%. Ion suppression was observed with urine concentrations >10%. Mean levels of saccharin and resveratrol-3-O-sulfate in urine were 5.481 ± 4.359 and 3.440 ± 4.160 nmol L, respectively. We developed and validated a method to measure saccharin and -resveratrol metabolites in urine to objectively corroborate the consumption of SQ-LNS for the first time in nutrition intervention studies.
糖精和白藜芦醇被纳入小剂量脂质营养补充剂（SQ-LNS）中，以作为营养干预研究中摄入量的标志物进行评估。47 名健康女性单次摄入含有 8.6 毫克糖精或 5 毫克白藜芦醇的补充剂，并在 4 小时内收集尿液。建立了一种快速的 11 分钟方法，采用多重反应监测和超高效液相色谱与三重四极杆质谱联用，同时测定尿液中的糖精和白藜芦醇代谢物。该方法的线性动态范围为 3 至 1000 ng/mL，相关系数为 0.999，定量下限为 15.28 至 53.03 ng/mL。样品制备简单，仅需稀释，平均回收率为 97.8%。当尿液浓度>10%时，观察到离子抑制。尿液中糖精和白藜芦醇-3-O-硫酸盐的平均水平分别为 5.481±4.359 和 3.440±4.160 nmol/L。我们开发并验证了一种方法来测量尿液中的糖精和白藜芦醇代谢物，这是首次在营养干预研究中客观证实 SQ-LNS 的摄入量。
