Ayuso R, Polo F, Carreira J
Departamento de Investigación Biológica, Alergia e Inmunologia Abelló S.A., Madrid, Spain.
Mol Immunol. 1988 Jan;25(1):49-56. doi: 10.1016/0161-5890(88)90089-2.
A component of Parietaria judaica pollen extract, previously identified as the major allergen, then reported as Pj10 and hereafter denominated Par j I has been isolated by a combination of 65% ammonium sulphate salt precipitation and gel filtration and an Ultrogel AcA54 column. The purified allergen appeared essentially homogeneous on gel filtration HPLC. The mol. wt of Par j I was estimated by electrophoretic and chromatographic techniques. All results gave values in a range from 13 K to 25 K. Analysis in SDS-PAGE under reducing conditions revealed a broad band corresponding to a mol. wt of 10 K, which retained allergenicity when tested with a patients serum pool. CIE and CRIE patterns of the isolated Par j I displayed the two precipitating lines already reported as those exhibiting the highest IgE-binding ability. Par j I showed a specific allergenic activity about 10-fold higher than that of the whole extract and was demonstrated to be the major allergen of Parietaria judaica as assessed in 25 sensitive human sera.
墙草花粉提取物的一种成分，先前被鉴定为主要过敏原，后报道为Pj10，现命名为Par j I，已通过65%硫酸铵盐沉淀和凝胶过滤相结合的方法以及Ultrogel AcA54柱进行分离。纯化后的过敏原在凝胶过滤HPLC上基本呈现均一性。通过电泳和色谱技术对Par j I的分子量进行了估计。所有结果给出的值在13K至25K的范围内。在还原条件下进行的SDS-PAGE分析显示出一条对应分子量为10K的宽带，用患者血清池检测时该条带保留了致敏性。分离出的Par j I的CIE和CRIE图谱显示出两条沉淀线，已报道这两条线具有最高的IgE结合能力。Par j I的特异性致敏活性比全提取物高约10倍，并且在25份敏感的人血清中评估时被证明是墙草的主要过敏原。
