Dreborg S, Basomba A, Einarsson R
Allergol Immunopathol (Madr). 1986 Nov-Dec;14(6):499-508.
To investigate the biological activity of P. judaica and P. officinalis allergen preparations, 36 Spanish patients were included in a biological standardization (BS) trial. For both species the concentration corresponding to 1000 biological units/ml (BU/ml) was found to be about 2 micrograms dry weight/ml (d.w./ml). The allergenic activity per microgram d.w. was about the same as reported for other important weed pollen extracts. The physico-chemical and immunological properties of the purified Parietaria judaica and Parietaria officinalis pollen allergen preparations used for biological standardization were investigated. The protein content was determined to 38% and 23% for P. judaica and P. officinalis respectively, while the carbohydrate content was 25% in both weed preparations. More than 40 antigens and at least 10 different allergens were detected by crossed immuno electrophoresis/crossed radio immunoelectrophoresis (CIE/CRIE) analyses. SDS-gPAGE followed by electroblotting showed important allergens between 10,000 and 75,000 d for both species. The crossreactivity as measured by skin prick test, RAST and CRIE was high.
为研究犹太墙草和药用墙草过敏原制剂的生物活性，36名西班牙患者被纳入一项生物标准化（BS）试验。对于这两个物种，发现对应于1000生物单位/毫升（BU/毫升）的浓度约为2微克干重/毫升（d.w./毫升）。每微克干重的致敏活性与其他重要杂草花粉提取物的报道大致相同。对用于生物标准化的纯化犹太墙草和药用墙草花粉过敏原制剂的物理化学和免疫学特性进行了研究。犹太墙草和药用墙草的蛋白质含量分别测定为38%和23%，而两种杂草制剂中的碳水化合物含量均为25%。通过交叉免疫电泳/交叉放射免疫电泳（CIE/CRIE）分析检测到40多种抗原和至少10种不同的过敏原。SDS - gPAGE随后进行电印迹显示，两个物种在10,000至75,000道尔顿之间存在重要过敏原。通过皮肤点刺试验、RAST和CRIE测量的交叉反应性很高。
