Menzies Donna J, Ang Andrew, Thissen Helmut, Evans Richard A
Commonwealth Scientific and Industrial Research Organisation (CSIRO) Manufacturing, Clayton, VIC 3169, Australia.
Faculty of Science, Engineering and Technology, Swinburne University, Hawthorn, VIC 3122, Australia.
ACS Biomater Sci Eng. 2017 May 8;3(5):793-806. doi: 10.1021/acsbiomaterials.7b00038. Epub 2017 Mar 29.
New and improved bone-contacting medical devices are required to provide excellent bioactivity at the biointerface. Here, we have used coatings based on prebiotic chemistry inspired polymerization of aminomalonitrile (AMN) in combination with comonomers 3,4-di- and 3,4,5-trihydroxybenzaldehyde (DHBA and THBA). The comonomers were incorporated into the AMN coatings to enhance polymerization kinetics, adhesive properties, metal binding efficacy, and human mesenchymal stem cell (hMSC) response. Incorporation of DHBA and THBA as separate comonomers enhanced the polymerization kinetics compared to that of AMN polymerization alone, with 30 mol % THBA (30T) resulting in a 6-fold increase in thickness over 24 h. Furthermore, the adhesion of AMN coatings to silicon was enhanced when copolymerized with the HBA monomers, where the interfacial adhesion of the 30T coating was increased 20-fold. The ability of the coatings to incorporate zinc ions was investigated, and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that incorporating 30T increased the binding efficiency 4-fold compared to that of AMN alone. The attachment, proliferation, and morphology of human mesenchymal stem cells (hMSC) on these coatings was investigated and reported. Finally, the utility of the coatings as osteogenic support matrices via the induced osteogenic differentiation of hMSCs is reported. The AMN and 30T coatings resulted in the greatest efficiency of osteogenic differentiation, as measured by intracellular ALP activity and mineralization. Incorporation of zinc had a stimulatory effect on hMSC proliferation with 30T coatings, while enhanced mineralization was observed with the zinc functionalized AMN and 30T coatings. This study highlights the potential of prebiotic chemistry inspired coatings in biomedical applications.
新型且改良的骨接触医疗设备需要在生物界面提供出色的生物活性。在此，我们使用了基于益生元化学启发的氨基丙二腈（AMN）聚合涂层，并结合了共聚单体3,4 - 二羟基苯甲醛和3,4,5 - 三羟基苯甲醛（DHBA和THBA）。将共聚单体引入AMN涂层以增强聚合动力学、粘附性能、金属结合效率以及人间充质干细胞（hMSC）反应。与单独的AMN聚合相比，将DHBA和THBA作为单独的共聚单体引入可增强聚合动力学，30摩尔％的THBA（30T）在24小时内使涂层厚度增加了6倍。此外，当与HBA单体共聚时，AMN涂层与硅的粘附力增强，其中30T涂层的界面粘附力提高了20倍。研究了涂层结合锌离子的能力，X射线光电子能谱（XPS）分析表明，与单独的AMN相比，引入30T可使结合效率提高4倍。研究并报告了人间充质干细胞（hMSC）在这些涂层上的附着、增殖和形态。最后，报告了通过诱导hMSC的成骨分化，这些涂层作为成骨支持基质的效用。通过细胞内碱性磷酸酶活性和矿化作用测定，AMN和30T涂层导致成骨分化效率最高。锌的引入对30T涂层的hMSC增殖有刺激作用，而锌功能化的AMN和30T涂层则观察到矿化增强。这项研究突出了益生元化学启发涂层在生物医学应用中的潜力。
