Azeem A, Marani L, Fuller K, Spanoudes K, Pandit A, Zeugolis D I
Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, and ‡Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, and Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.
ACS Biomater Sci Eng. 2017 Jul 10;3(7):1304-1312. doi: 10.1021/acsbiomaterials.6b00206. Epub 2016 Sep 22.
Biomimetic tissue engineering aspires to develop bioinspired implantable devices that would positively interact with the host. Given that glycosaminoglycans are involved in many physiological processes, whereas their deprivation is associated with pathophysiologies, functionalization of implantable devices with natural and/or synthetic carbohydrate moieties is at the forefront of scientific research and industrial innovation. Herein, we venture to assess the influence of various concentrations (0.01%, 0.1%, 1%) of hyaluronic acid and Ficoll on the structural, thermal, biomechanical and biological (human osteoblasts) properties of electrospun poly(lactic--glycolic acid) fibers. The addition of hyaluronic acid and Ficoll reduced the fiber diameter, with the 1% hyaluronic acid exhibiting the smallest fibers diameter ( < 0.001). Neither the addition of hyaluronic acid nor the addition Ficoll significantly affected the onset and peak temperatures ( > 0.05). All hyaluronic acid and Ficoll treatments significantly reduced stress at break, strain at break and elastic modulus values ( < 0.001). Hyaluronic acid and Ficoll did not affect osteoblast viability and metabolic activity temperatures ( > 0.05); the 1% hyaluronic acid and Ficoll significantly increased ( < 0.001) osteoblast proliferation at day 21. By day 21, the 1% hyaluronic acid and 1% Ficoll fibers showed the highest alkaline phosphatase activity and calcium deposition. At day 21, osteocalcin was not detected, whereas osteopontin was detected on all samples. Collectively, our data clearly illustrate the biological benefit of nonsulfated polysaccharides as functionalization molecules.
仿生组织工程致力于开发能与宿主产生积极相互作用的受生物启发的可植入装置。鉴于糖胺聚糖参与许多生理过程，而其缺失与病理生理学相关，用天然和/或合成碳水化合物部分对可植入装置进行功能化处于科学研究和产业创新的前沿。在此，我们尝试评估不同浓度（0.01%、0.1%、1%）的透明质酸和聚蔗糖对电纺聚乳酸-乙醇酸纤维的结构、热、生物力学和生物学（人成骨细胞）特性的影响。透明质酸和聚蔗糖的添加降低了纤维直径，1%透明质酸表现出最小的纤维直径（<0.001）。透明质酸的添加和聚蔗糖的添加均未显著影响起始温度和峰值温度（>0.05）。所有透明质酸和聚蔗糖处理均显著降低了断裂应力、断裂应变和弹性模量值（<0.001）。透明质酸和聚蔗糖不影响成骨细胞活力和代谢活性温度（>0.05）；1%透明质酸和聚蔗糖在第21天显著增加了成骨细胞增殖（<0.001）。到第21天，1%透明质酸和1%聚蔗糖纤维显示出最高的碱性磷酸酶活性和钙沉积。在第21天，未检测到骨钙素，而在所有样品上均检测到骨桥蛋白。总体而言，我们的数据清楚地说明了非硫酸化多糖作为功能化分子的生物学益处。
