Dong Xia, Wei Chang, Chen Hongli, Qin Jingwen, Liang Jie, Kong Deling, Liu Tianjun, Lv Feng
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, PR China.
School of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan, 453003, PR China.
ACS Biomater Sci Eng. 2016 Nov 14;2(11):2001-2010. doi: 10.1021/acsbiomaterials.6b00403. Epub 2016 Sep 15.
Real-time tracking of a drug delivery system and its therapeutic effects in vivo are crucial to designing a novel pharmaceutical system and revealing the mechanism of drug therapy. Multispectral fluorescence imaging can locate the drug and carrier simultaneously without interference. This advanced method enables the tracking of a drug delivery system. Herein, a doxorubicin (Dox) loaded zinc phthalocyanine incorporated hydrogel was developed as a dual fluorescent drug delivery system to monitor the release of the drug and the degradation of the carrier. An injectable thermosensitive hydrogel based on a four-arm poly(ethylene glycol) (PEG)-poly(ε-Caprolactone) (PCL) copolymer was prepared and characterized with a zinc phthalocyanine core as the drug carrier. The hydrogel degradation and drug delivery in vivo were tracked by a multispectral fluorescence imaging system in nude mice bearing hepatic tumors. Moreover, the real-time tumor inhibition progress was tracked in vivo for 18 days by bioluminescence imaging. A multispectral analysis can separate the fluorescence signals from the drug and carrier in the Dox loaded hydrogel and provide their location in the tumor tissue. The drug release and hydrogel degradation can be drastically tracked respectively without mutual interference. The fluorescence imaging results reveal improved tumor inhibitory effects of the Dox loaded hydrogel. Optical imaging allows for visible tracking of the entire drug delivery process. The Dox loaded phthalocyanine incorporated thermosensitive hydrogel is a potential visible drug delivery system for tumor therapy.
实时跟踪药物递送系统及其体内治疗效果对于设计新型药物系统和揭示药物治疗机制至关重要。多光谱荧光成像可同时定位药物和载体，且不受干扰。这种先进方法能够对药物递送系统进行跟踪。在此，开发了一种负载阿霉素（Dox）的酞菁锌掺入水凝胶作为双荧光药物递送系统，以监测药物释放和载体降解。制备了一种基于四臂聚乙二醇（PEG）-聚己内酯（PCL）共聚物的可注射热敏水凝胶，并以酞菁锌为药物载体进行了表征。通过多光谱荧光成像系统在荷肝癌裸鼠体内跟踪水凝胶的降解和药物递送。此外，通过生物发光成像在体内实时跟踪肿瘤抑制进展18天。多光谱分析可分离负载Dox水凝胶中药物和载体的荧光信号，并提供它们在肿瘤组织中的位置。可分别显著跟踪药物释放和水凝胶降解，且互不干扰。荧光成像结果显示负载Dox的水凝胶具有更好的肿瘤抑制效果。光学成像能够对整个药物递送过程进行可视化跟踪。负载Dox的酞菁掺入热敏水凝胶是一种潜在的用于肿瘤治疗的可视化药物递送系统。
