Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom, 73000, Thailand.
AAPS PharmSciTech. 2023 Jun 12;24(5):135. doi: 10.1208/s12249-023-02593-w.
Carbon-based nanoparticles (CNPs) are a new type of interesting nanomaterials applied in various pharmaceutical fields due to their outstanding biocompatible properties. Novel pH-sensitive CNPs were rapidly synthesized within 1 min by microwave-assisted technique for doxorubicin (DOX) delivery into five cancer cell lines, including breast cancer (BT-474 and MDA-MB-231 cell lines), colon cancer (HCT and HT29 cell lines), and cervical cancer (HeLa cell lines). CNPs and DOX-loaded CNPs (CNPs-DOX) had nano-size of 11.66 ± 2.32 nm and 43.24 ± 13.25 nm, respectively. DOX could be self-assembled with CNPs in phosphate buffer solution at pH 7.4 through electrostatic interaction, exhibiting high loading efficiency at 85.82%. The release of DOX from CNPs-DOX at pH 5.0, often observed in the tumor, was nearly two times greater than the release at physiological condition pH 7.4. Furthermore, the anticancer activity of CNPs-DOX was significantly enhanced compared to free DOX in five cancer cell lines. CNPs-DOX could induce cell death through apoptosis induction in MDA-MB-231 cells. The findings revealed that CNPs-DOX exhibited a promising pH-sensitive nano-system as a drug delivery carrier for cancer treatment.
碳基纳米粒子（CNPs）是一种新型的有趣纳米材料，由于其出色的生物相容性而被应用于各个制药领域。通过微波辅助技术，在 1 分钟内快速合成了新型 pH 敏感的 CNPs，用于将阿霉素（DOX）递送至五种癌细胞系，包括乳腺癌（BT-474 和 MDA-MB-231 细胞系）、结肠癌（HCT 和 HT29 细胞系）和宫颈癌（HeLa 细胞系）。CNPs 和载 DOX 的 CNPs（CNPs-DOX）的粒径分别为 11.66±2.32nm 和 43.24±13.25nm。DOX 可以通过静电相互作用在 pH 7.4 的磷酸盐缓冲溶液中自组装到 CNPs 中，载药效率高达 85.82%。在 pH 5.0（通常在肿瘤中观察到的条件）下，DOX 从 CNPs-DOX 的释放速度比在生理条件 pH 7.4 下快近两倍。此外，与游离 DOX 相比，CNPs-DOX 在五种癌细胞系中的抗癌活性显著增强。CNPs-DOX 可以通过诱导 MDA-MB-231 细胞凋亡诱导细胞死亡。研究结果表明，CNPs-DOX 作为一种治疗癌症的药物递送载体，表现出一种有前途的 pH 敏感纳米系统。
