White Lewis P, Price Jessica S
Mycology Reference Laboratory, Public Health Wales, Microbiology Cardiff, Cardiff CF14 4XW, UK.
J Fungi (Basel). 2021 Jan 11;7(1):41. doi: 10.3390/jof7010041.
What was once just culture and microscopy the field of diagnostic mycology has significantly advanced in recent years and continues to incorporate novel assays and strategies to meet the changes in clinical demand. The emergence of widespread resistance to antifungal therapy has led to the development of a range of molecular tests that target mutations associated with phenotypic resistance, to complement classical susceptibility testing and initial applications of next-generation sequencing are being described. Lateral flow assays provide rapid results, with simplicity allowing the test to be performed outside specialist centres, potentially as point-of-care tests. Mycology has responded positively to an ever-diversifying patient population by rapidly identifying risk and developing diagnostic strategies to improve patient management. Nowadays, the diagnostic repertoire of the mycology laboratory employs classical, molecular and serological tests and should be keen to embrace diagnostic advancements that can improve diagnosis in this notoriously difficult field.
曾经仅依靠培养和显微镜检查的诊断真菌学领域近年来取得了显著进展，并不断纳入新的检测方法和策略，以满足临床需求的变化。抗真菌治疗广泛耐药性的出现促使一系列针对与表型耐药相关突变的分子检测方法得以开发，以补充传统的药敏试验，并且正在描述下一代测序的初步应用。侧向流动分析能够快速得出结果，其操作简单，使得检测可在专科中心以外的地方进行，有可能作为即时检验。真菌学通过快速识别风险并制定诊断策略以改善患者管理，对日益多样化的患者群体做出了积极回应。如今，真菌学实验室的诊断方法包括经典、分子和血清学检测，并且应该积极采用能够改善这一极其困难领域诊断的先进技术。
