Lin Liqiong, Shen Kaiwei, Xiao Lili, Lin Yu, Feng Eryou, Zhang Yiyuan
Department of Orthopedics, Fuzhou Second Hospital, Fuzhou, PR China.
Fujian Provincial Clinical Medical Research Center for First Aid and Rehabilitation in Orthopaedic Trauma, Fuzhou, PR China.
Access Microbiol. 2023 Oct 26;5(10):000425. doi: 10.1099/acmi.0.000425. eCollection 2023.
Periprosthetic joint infection (PJI) is a serious complication after total knee arthroplasty. Fungal infections are prone to biofilm formation, which makes it hard to diagnose and clarify the pathogenic species.
This case study provides evidence of a novel PJI pathogen that is otherwise difficult to detect using conventional methods. A patient was reviewed with persistent postoperative pain, swelling and eventually drainage around the left knee after undergoing a bilateral total knee arthroplasty 2 years previously for progressive osteoarthritis. By using metagenomic shotgun sequencing to analyse both bacterial and fungal agent sequences, we were able to identify fungal strains of , a rarely reported and difficult-to-culture PJI pathogen.
Metagenomic shotgun sequencing enables the detection of difficult-to-detect pathogens and the formulation of treatment recommendations for fungal infections with low positive rates based on gene content analysis.
人工关节周围感染（PJI）是全膝关节置换术后的一种严重并发症。真菌感染易于形成生物膜，这使得其难以诊断和明确致病菌种。
本病例研究提供了一种新型PJI病原体的证据，该病原体用传统方法难以检测。一名患者因进行性骨关节炎于2年前接受双侧全膝关节置换术，术后左膝持续疼痛、肿胀，最终出现渗液。通过使用宏基因组鸟枪法测序分析细菌和真菌病原体序列，我们能够鉴定出一种很少报道且难以培养的PJI病原体——[具体真菌菌株名称未给出]的真菌菌株。
宏基因组鸟枪法测序能够检测难以检测的病原体，并基于基因含量分析为阳性率较低的真菌感染制定治疗建议。
