Laboratório de Processos Biotecnológicos (LABIOTEC), Programa de Pós-graduação em Ciência e Tecnologia de Alimentos (PPGCTA), Instituto de Tecnologia (ITEC), Universidade Federal do Pará (UFPA), Belém 66075-110, Brazil.
Laboratório Adolpho Ducke, Coordenação de Botânica, Museu Paraense Emílio Goeldi, Av. Perimetral, 1900, Terra Firme, Belém 66077-830, Brazil.
Molecules. 2021 Jan 11;26(2):344. doi: 10.3390/molecules26020344.
This study aimed to identify the volatile compounds in the fermented and dried cocoa beans conducted with three distinct inoculants of yeast species due to their high fermentative capacity: , , the mixture in equal proportions 1:1 of both species, and a control fermentation (with no inoculum application). Three starter cultures of yeasts, previously isolated and identified in cocoa fermentation in the municipality of Tomé-Açu, Pará state, Brazil. The seeds with pulp were removed manually and placed in wooden boxes for the fermentation process that lasted from 6 to 7 days. On the last day of fermentation, the almonds were packaged properly and placed to dry (36 °C), followed by preparation for the analysis of volatile compounds by GC-MS technique. In addition to the control fermentation, a high capacity for the formation of desirable compounds in chocolate by the inoculants with was observed, which was confirmed through multivariate analyses, classifying these almonds with the highest content of aldehydes, esters, ketones and alcohols and low concentration of off-flavours. We conclude that the addition of mixed culture starter can be an excellent alternative for cocoa producers, suggesting obtaining cocoa beans with desirable characteristics for chocolate production, as well as creating a product identity for the producing region.
本研究旨在鉴定三种不同酵母接种剂(具有高发酵能力)发酵和干燥可可豆产生的挥发性化合物:、、两种菌等量混合 1:1 的比例,以及对照发酵(不接种)。三种酵母启动培养物,先前在巴西帕拉州托梅-阿苏市的可可发酵中分离和鉴定。手动去除果肉的种子,并放在木箱中进行发酵过程,持续 6-7 天。在发酵的最后一天,杏仁被妥善包装并进行干燥(36°C),然后准备通过 GC-MS 技术分析挥发性化合物。除了对照发酵外,接种剂 产生了大量对巧克力形成有吸引力的化合物,通过多元分析证实了这一点,这些杏仁具有最高含量的醛、酯、酮和醇,以及低浓度的异味。我们得出结论,添加混合培养物启动物可以成为可可生产者的绝佳选择,建议获得具有理想巧克力生产特性的可可豆,并为生产地区创造产品特色。