Xiamen Key Laboratory of Marine Medicinal Natural Products Resources, Xiamen Medical College, Xiamen, 361023, People's Republic of China.
Fujian Province Universities and Colleges Engineering Research Center for Marine Biomedical Resources Utilization, Xiamen Medical College, Xiamen, 361023, People's Republic of China.
Sci Rep. 2021 Jan 13;11(1):1040. doi: 10.1038/s41598-020-80198-0.
Cisplatin, metformin, and quercetin are all reliable anticancer drugs. However, it is unclear how effective their different combination regimens are on the growth of nasopharyngeal carcinoma cell line Sune-1 and subcutaneous xenograft in nude mice. This study evaluated the effects of single-drug, two-drug, and three-drug simultaneous or sequential combined application of these drugs on the growth of Sune-1 cells and subcutaneous xenograft tumors in nude mice. The results showed that the different combination regimens of cisplatin, metformin and quercetin all had significant inhibitory effects on the proliferation of Sune-1 cells and the growth of subcutaneous xenografts in nude mice (P < 0.01), and the inhibition rate of the three drugs simultaneous combined application was significant Higher than the two-drug combination or single-drug application (P < 0.05), the contribution level of each drug in the three-drug combination application from high to low were cisplatin > metformin > quercetin. In summary, our results indicate that the simultaneous combination of cisplatin, metformin, and quercetin may synergistically inhibit the growth of Sune-1 cells and subcutaneous xenografts in nude mice through their different anticancer mechanisms, which may be clinically refractory and provide reference for chemotherapy of patients with recurrent nasopharyngeal carcinoma.
顺铂、二甲双胍和槲皮素都是可靠的抗癌药物。然而，它们不同联合方案对鼻咽癌细胞系 Sune-1 生长和裸鼠皮下异种移植的影响尚不清楚。本研究评估了这些药物的单药、两药和三药同时或序贯联合应用对 Sune-1 细胞生长和裸鼠皮下异种移植瘤生长的影响。结果表明，顺铂、二甲双胍和槲皮素的不同联合方案均对 Sune-1 细胞的增殖和裸鼠皮下异种移植瘤的生长有显著抑制作用（P<0.01），且三药同时联合应用的抑制率明显高于两药联合或单药应用（P<0.05），三药联合应用中各药的贡献水平从高到低依次为顺铂>二甲双胍>槲皮素。综上所述，我们的结果表明，顺铂、二甲双胍和槲皮素的同时联合应用可能通过不同的抗癌机制协同抑制 Sune-1 细胞和裸鼠皮下异种移植瘤的生长，这可能为临床难治性患者提供参考，为复发性鼻咽癌患者的化疗提供参考。
