Department of Biotechnology, Faculty of Life Sciences and Biotechnology, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima, 729-0292, Japan.
Yeast. 2021 May;38(5):326-335. doi: 10.1002/yea.3549. Epub 2021 Jan 29.
We have previously isolated heterothallic haploid strains from original homothallic diploids of two yeast species in the family Saccharomycetaceae. In this study, heterothallic haploid strains were isolated from an original homothallic diploid of Saccharomyces kudriavzevii type strain, followed by investigation of sexual interactions among these yeast strains, in addition to S. cerevisiae laboratory strains. It has been shown that prezygotic reproductive isolation was observed between Kazachstania naganishii and S. cerevisiae with α-factor mating pheromones representing crossaction with each other beyond the genus boundary. Using heterothallic strains, postzygotic reproductive isolation system was shown to reside in the genus Saccharomyces by mass mating and cell-cell contact experiments. In mass mating experiments, crossaction of α-factor and a-factor mating pheromones and sexual agglutination effectively occurred beyond species boundaries among S. kudriavzevii, S. paradoxus, and S. cerevisiae. When the fates of cell-cell pairs from these Saccharomyces yeast species were systematically chased one by one, interspecific F1 hybrids were effectively produced, while sporulations were partially prohibited, with spore germination perfectly blocked in the hybrids. These results indicated that postzygotic reproductive isolation definitively resides among these Saccharomyces yeast species and that disorder of chromosome organization had to some extent occurred in interspecific F1 hybrids.
我们之前已经从酿酒酵母科的两个酵母物种的原始同型二倍体中分离出异型单倍体菌株。在这项研究中，从萨奇酵母原始同型二倍体的型菌株中分离出异型单倍体菌株，然后研究这些酵母菌株之间的性相互作用，以及酿酒酵母实验室菌株。结果表明，Kazachstania naganishii 和酿酒酵母之间存在预配子生殖隔离，用α因子交配信息素来表示彼此之间的交叉作用超过了属的边界。使用异型菌株，通过大量交配和细胞-细胞接触实验表明，在属 Saccharomyces 中存在后配子生殖隔离系统。在大量交配实验中，α因子和 a 因子交配信息素的交叉作用以及性聚集在萨奇酵母、酿酒酵母和 S. paradoxus 等物种之间有效地发生。当系统地追踪这些酿酒酵母物种的细胞-细胞对的命运时，有效地产生了种间 F1 杂种，而部分禁止了孢子形成，杂种中的孢子萌发被完全阻断。这些结果表明，后配子生殖隔离明确存在于这些酿酒酵母物种之间，并且种间 F1 杂种中的染色体组织紊乱发生在一定程度上。
