Franco Filipe Nogueira, de Cassia Cardoso Luciana, Moura Silva Bárbara Néllita, Rodrigues de Araújo Glaucy, Chaves Miriam Martins
Biochemistry Laboratory of Aging and Department of Biochemistry and Immunology, Correlated Diseases, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte/MG, Brazil.
Curr Aging Sci. 2023;16(1):56-64. doi: 10.2174/1874609815666220511143843.
One of the most studied theories about aging comes from the accumulation of free radical generation, leading to oxidative stress. Resveratrol (RSV) is a polyphenolic compound that has been shown to act as an antioxidant in medical practice.
To verify the antioxidant action of resveratrol (and its correlation with aging) in leukocytes from donors of different ages, mainly through the analysis of the three main enzymes of the antioxidant complex and the analysis of the SIRT1 signaling pathway.
Luminol-dependent chemiluminescence assay was used to evaluate ROS and SIRT1. Antioxidant enzymes were evaluated by commercial kits. *p<0.05.
In all age groups, there was a reduction in reactive oxygen species (ROS) in cells stimulated with RSV. There was a positive correlation between its antioxidant effect and donor age. In younger individuals (20-39 years old), there was an increase in catalase activity in cells exposed to RSV. In the older groups (40-59 years old and 60-80 years old), RSV was able to increase the activity of the enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx). Through the analysis of SIRT1 it was possible to observe a silencing of the pathway in leukocytes treated with RSV during aging.
RSV showed antioxidant activity in all age groups, although more pronounced in younger individuals. One of the mechanisms of action of the RSV is due to the increase in the activity of antioxidant enzymes, which varies according to the individual's age, especially through the modulation of important antioxidant pathways.
关于衰老研究最多的理论之一源于自由基生成的积累，导致氧化应激。白藜芦醇（RSV）是一种多酚化合物，在医学实践中已被证明具有抗氧化作用。
主要通过分析抗氧化复合物的三种主要酶以及SIRT1信号通路，验证白藜芦醇在不同年龄供体白细胞中的抗氧化作用（及其与衰老的相关性）。
采用鲁米诺依赖性化学发光法评估活性氧（ROS）和SIRT1。通过商业试剂盒评估抗氧化酶。*p<0.05。
在所有年龄组中，用RSV刺激的细胞中活性氧（ROS）均减少。其抗氧化作用与供体年龄呈正相关。在较年轻个体（20 - 39岁）中，暴露于RSV的细胞中过氧化氢酶活性增加。在较年长组（40 - 59岁和60 - 80岁）中，RSV能够增加超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。通过对SIRT1的分析，可以观察到衰老过程中用RSV处理的白细胞中该信号通路的沉默。
RSV在所有年龄组中均显示出抗氧化活性，尽管在较年轻个体中更为明显。RSV的作用机制之一是由于抗氧化酶活性的增加，这根据个体年龄而有所不同，尤其是通过对重要抗氧化途径的调节。
