Sexing Technologies, 22575 State Hwy 6 S, Navasota, TX, 77868, USA.
Sexing Technologies, 22575 State Hwy 6 S, Navasota, TX, 77868, USA.
Theriogenology. 2021 Mar 1;162:67-73. doi: 10.1016/j.theriogenology.2020.12.028. Epub 2021 Jan 3.
Flow cytometry sperm sex-sorting based on the relative DNA difference between X- and Y-chromosome bearing populations is an established method that has allowed the production of pre-sexed offspring in a multitude of species and has been a commercial success in cattle production for the past twenty years. Several improvements to the technology and the processing methods have increased the sorting efficiency of ejaculates and the post-thaw quality of sex-sorted sperm, allowing for the fertility gap between conventional (non-sorted) and SexedULTRA™ sex-sorted sperm to be bridged. Small ruminant industries are now progressively testing sex-sorted sperm for application in their specific niches and environments. A review of the key advances and the recent developments in caprine, ovine and cervine sperm sex-sorting technology are described in this publication.
基于 X 染色体和 Y 染色体携带群体之间的相对 DNA 差异的流式细胞术精子性别分选是一种成熟的方法，已经在多种物种中实现了预先性别的后代生产，并在过去二十年中在牛生产中取得了商业成功。该技术和处理方法的几项改进提高了精液的分选效率和分选后精子的解冻质量，缩小了传统（非分选）和 SexedULTRA™ 性别分选精子之间的生育差距。小反刍动物产业现在正在逐步测试性别分选精子在其特定利基市场和环境中的应用。本文综述了山羊、绵羊和鹿精液性别分选技术的关键进展和最新发展。
