Ziemba Alexis M, Lane Keith P, San Segundo Ignacio M, D'Amato Anthony R, Mason Andrew K, Sexton Ryan J, Casajus Hubert, Gross Richard A, Corr David T, Gilbert Ryan J
Center for Biotechnology and Interdisciplinary Sciences, Rensselaer Polytechnic Institute, 1623 15th Street, Troy, New York 12180, United States.
ACS Biomater Sci Eng. 2018 May 14;4(5):1491-1497. doi: 10.1021/acsbiomaterials.8b00013. Epub 2018 Apr 4.
Electrospun poly-l-lactic acid (PLLA) fiber scaffolds are used to direct axonal extension in neural engineering models. We aimed to improve the efficacy of these fibers in promoting neurite outgrowth by altering surface topography and reducing fiber elastic modulus through the incorporation of a compatibilized blend, poly-l-lactic acid-poly(pentadecalactone) (PLLA-PPDL) into the solution prior to electrospinning. PLLA+PLLA-PPDL fibers had a larger diameter, increased surface nanotopography, and lower glass transition temperature than PLLA fibers but had similar mechanical properties. Increases in neurite outgrowth on PLLA+PLLA-PPDL fibers were observed, potentially due to the significantly increased diameter and surface coverage with nanotopography. Ultimately, these results suggest that greater electrospun fiber diameter and surface topography may contribute to increases in neurite outgrowth.
静电纺聚左旋乳酸（PLLA）纤维支架用于神经工程模型中引导轴突延伸。我们旨在通过改变表面形貌并在静电纺丝前将相容共混物聚左旋乳酸-聚（十五内酯）（PLLA-PPDL）加入溶液中以降低纤维弹性模量，来提高这些纤维促进神经突生长的功效。与PLLA纤维相比，PLLA+PLLA-PPDL纤维直径更大、表面纳米形貌增加且玻璃化转变温度更低，但机械性能相似。观察到PLLA+PLLA-PPDL纤维上神经突生长增加，这可能是由于直径显著增加以及纳米形貌的表面覆盖率增加所致。最终，这些结果表明更大的静电纺纤维直径和表面形貌可能有助于神经突生长的增加。
