Parlane Natalie A, Gupta Sandeep K, Rubio-Reyes Patricia, Chen Shuxiong, Gonzalez-Miro Majela, Wedlock D Neil, Rehm Bernd H A
AgResearch, Hopkirk Research Institute, Palmerston North 4442, New Zealand.
Institute of Fundamental Sciences, Massey University, Private Bag 11222, Palmerston North 4442, New Zealand.
ACS Biomater Sci Eng. 2017 Dec 11;3(12):3043-3057. doi: 10.1021/acsbiomaterials.6b00355. Epub 2016 Sep 13.
Polyhydroxyalkanoates (PHAs) are biological polyesters that can be naturally produced by a range of bacteria as water-insoluble inclusions composed of a PHA core coated with PHA synthesis, structural, and regulatory proteins. These naturally self-assembling shell-core particles have been recently conceived as biomaterials that can be bioengineered as biologically active beads for medical applications. Protein engineering of PHA-associated proteins enabled the production of PHA-protein assemblies exhibiting biologically active protein-based functions relevant for applications as vaccines or diagnostics. Here we provide an overview of the recent advances in bioengineering of PHA particles toward the display of biomedically relevant protein functions such as selected disease-specific antigens as diagnostic tools or for the design of particulate subunit vaccines against infectious diseases such as tuberculosis, meningitis, pneumonia, and hepatitis C.
聚羟基脂肪酸酯（PHA）是一类生物聚酯，可由多种细菌天然产生，形成水不溶性内含物，其核心为PHA，表面包裹着PHA合成、结构和调节蛋白。这些天然自组装的核壳颗粒最近被视为生物材料，可通过生物工程改造为具有生物活性的珠子用于医学应用。对PHA相关蛋白进行蛋白质工程改造，能够生产出具有基于蛋白质的生物活性功能的PHA-蛋白质组装体，这些功能与疫苗或诊断应用相关。在此，我们概述了PHA颗粒生物工程的最新进展，这些进展旨在展示与生物医学相关的蛋白质功能，例如将选定的疾病特异性抗原作为诊断工具，或设计针对结核病、脑膜炎、肺炎和丙型肝炎等传染病的颗粒亚单位疫苗。
