在 TGF-β1 的存在下，杠柳毒苷 VI 促进人骨髓间充质干细胞向髓核样细胞分化。

In the presence of TGF-β1, Asperosaponin VI promotes human mesenchymal stem cell differentiation into nucleus pulposus like- cells.

机构信息

Department of Spine Surgery, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, Jiangsu, China.

出版信息

BMC Complement Med Ther. 2021 Jan 14;21(1):32. doi: 10.1186/s12906-020-03169-y.

BACKGROUND

The regeneration of nucleus pulposus (NP) cells is an effective method to prevent intervertebral disc degeneration (IVDD). In this study, we investigated the role of Asperosaponin VI (ASA VI), isolated from a traditional Chinese medicine (TCM), the root of Dipsacus asper Wall, in promoting human mesenchymal stem cell (HMSC) proliferation and differentiation into NP-like cells and explored the possible mechanism of action.

METHODS

The effects of ASA VI on HMSC viability and proliferation were determined by the XTT method and EDU staining. Then, Real-time qPCR, immunocytochemistry and immunofluorescence assays were used to measure the effect of ASA VI on the expression of extracellular matrix (ECM) components, such as COL2A1, aggrecan, SOX9, KRT19, PAX1, and glycosaminoglycans (GAGs), in NP cells. In addition, Western blot assay was used to measure the expression of p-ERK1/2 and p-smad2/3.

RESULTS

ASA VI was able to promote the proliferation and differentiation of HMSCs into NP-like cells, and the optimum concentration was 1 mg/L. Western blot assay indicated that the possible mechanism might be related to the activation of p-ERK1 / 2 and p-Smad2 / 3.

CONCLUSIONS

ASA VI can promote the proliferation and differentiation of HMSCs into NP-like cells, which can potentially be used as a treatment for IVDD.

背景

修复髓核（NP）细胞是预防椎间盘退变（IVDD）的有效方法。本研究从传统中药（TCM）川续断的根中分离出asperosaponin VI（ASA VI），研究其在促进人间充质干细胞（HMSC）增殖和分化为 NP 样细胞中的作用，并探讨其可能的作用机制。

方法

XTT 法和 EDU 染色法检测 ASA VI 对 HMSC 活力和增殖的影响。然后，采用实时 qPCR、免疫细胞化学和免疫荧光法检测 ASA VI 对 NP 细胞细胞外基质（ECM）成分 COL2A1、聚集蛋白聚糖、SOX9、KRT19、PAX1 和糖胺聚糖（GAGs）表达的影响。此外，Western blot 检测 p-ERK1/2 和 p-smad2/3 的表达。