Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading - Harborne Building, Reading, RG6 6AS, UK.
Department of Life Sciences, Faculty of Science and Engineering, Manchester Metropolitan University, John Dalton Building, Manchester, M1 5GD, UK.
Sci Rep. 2021 Jan 14;11(1):1473. doi: 10.1038/s41598-020-80373-3.
Maternal over-nutrition increases the risk of diabetes and cardiovascular events in offspring. While prominent effects on cardiovascular health are observed, the impact on platelet physiology has not been studied. Here, we examined whether maternal high-fat diet (HF) ingestion affects the platelet function in lean and obese offspring. C57BL6/N mice dams were given a HF or control (C) diet for 8 weeks before and during pregnancy. Male and female offspring received C or HF diets for 26 weeks. Experimental groups were: C/C, dam and offspring fed standard laboratory diet; C/HF dam fed standard laboratory diet and offspring fed HF diet; HF/C and HF/HF. Phenotypic and metabolic tests were performed and blood collected for platelet studies. Compared to C/C, offspring HF groups were obese, with fat accumulation, hyperglycaemia and insulin resistance. Female offspring did not present platelet hyperactivity, hence we focused on male offspring. Platelets from HF/HF mice were larger, hyperactive and presented oxidative stress when compared to C/C. Maternal and offspring HF diet results in platelet hyperactivation in male mouse offspring, suggesting a novel 'double-hit' effect.
母体营养过剩会增加后代患糖尿病和心血管疾病的风险。虽然人们已经观察到它对心血管健康有显著影响，但对血小板生理学的影响尚未得到研究。在这里，我们研究了母体高脂肪饮食（HF）摄入是否会影响瘦素和肥胖后代的血小板功能。C57BL6/N 母鼠在怀孕前和怀孕期间接受 8 周的 HF 或对照（C）饮食。雄性和雌性后代接受 C 或 HF 饮食 26 周。实验组为：C/C，母鼠和后代喂养标准实验室饮食；C/HF 母鼠喂养标准实验室饮食，后代喂养 HF 饮食；HF/C 和 HF/HF。进行表型和代谢测试，并采集血液进行血小板研究。与 C/C 相比，HF 组后代肥胖，脂肪堆积，血糖升高和胰岛素抵抗。雌性后代的血小板没有表现出过度活跃，因此我们专注于雄性后代。与 C/C 相比，HF/HF 小鼠的血小板更大、更活跃，并且存在氧化应激。母体和后代的 HF 饮食导致雄性小鼠后代的血小板过度激活，提示存在一种新的“双重打击”效应。
