Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
Sci Rep. 2018 Apr 4;8(1):5607. doi: 10.1038/s41598-018-24010-0.
This study aimed to study the impact of a combination of maternal and post-weaning high-fat diets and whether resveratrol was beneficial. Sprague-Dawley dams were fed either chow or a high-fat diet, before mating, during pregnancy, and into lactation. At weaning, their offspring were randomly fed chow or a high-fat diet. Four experimental groups were generated: CC (maternal/postnatal chow diet), HC (maternal high-fat/postnatal chow diet), CH (maternal chow/postnatal high-fat diet), and HH (maternal/postnatal high-fat diet). A fifth group consisted of HH plus resveratrol. The 4 month-old offspring of HH group had higher body weight, higher levels of plasma triglycerides, leptin, angiotensin I and angiotensin II and abnormal intraperitoneal glucose tolerance test results, which fulfilled the features of metabolic syndrome. The dysregulation of the renin-angiotensin system was seen in multiple organs. Sirtuin 1 expression/abundance was reduced by a maternal/postnatal high-fat diet, in all the organs examined. Resveratrol ameliorated most of the features of metabolic syndrome and molecular alterations. The administration of a high-fat diet in both periods showed interactive metabolic effects in the plasma and many organs. Our results suggest that a maternal high-fat diet sensitizes offspring to the adverse effects of subsequent high-fat intake on multiple organs.
这项研究旨在研究母体和断奶后高脂肪饮食的组合的影响，以及白藜芦醇是否有益。在交配前、怀孕期间和哺乳期，Sprague-Dawley 母鼠分别喂食标准饮食或高脂肪饮食。断奶后，它们的后代被随机喂食标准饮食或高脂肪饮食。生成了四个实验组：CC（母体/产后标准饮食）、HC（母体高脂肪/产后标准饮食）、CH（母体标准饮食/产后高脂肪饮食）和 HH（母体/产后高脂肪饮食）。第五组由 HH 加白藜芦醇组成。HH 组 4 个月大的后代体重增加，血浆甘油三酯、瘦素、血管紧张素 I 和血管紧张素 II 水平升高，腹腔内葡萄糖耐量试验结果异常，符合代谢综合征的特征。在多个器官中观察到肾素-血管紧张素系统失调。在所有检查的器官中，高脂肪饮食都会降低 Sirtuin 1 的表达/丰度。白藜芦醇改善了代谢综合征和分子改变的大多数特征。在两个时期都给予高脂肪饮食会在血浆和许多器官中产生相互作用的代谢效应。我们的结果表明，母体高脂肪饮食使后代对随后高脂肪摄入对多个器官的不良影响敏感。
