异基因干细胞移植受者的T细胞免疫监测：基于全血的检测方法是否准备好挑战ELISPOT？
T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood-Based Assays Ready to Challenge ELISPOT?
作者信息
Lauruschkat Chris D, Page Lukas, Etter Sonja, Weis Philipp, Gamon Florian, Kraus Sabrina, Einsele Hermann, Wurster Sebastian, Loeffler Juergen
机构信息
Department of Internal Medicine II, University Hospital Wuerzburg, Wuerzburg, Germany.
Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
出版信息
Open Forum Infect Dis. 2020 Nov 8;8(1):ofaa547. doi: 10.1093/ofid/ofaa547. eCollection 2021 Jan.
We compared the feasibility of 4 cytomegalovirus (CMV)- and -reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.
我们比较了4种巨细胞病毒（CMV）反应性T细胞免疫测定方案在异基因干细胞移植受者中的可行性。虽然酶联免疫斑点法总体表现最佳，但在淋巴细胞减少不太严重的患者中，在后勤方面具有优势的全血检测法表现相当。在所有方案中，CMV诱导的γ干扰素反应相关性很强，并且与血清学高度一致。
Zotero 插件