Opt Lett. 2021 Jan 15;46(2):262-265. doi: 10.1364/OL.412993.
A blended FC-V-50 and TZ-001 polymer-based microdisk laser was fabricated by the ink-jet printing method and used for biosensing applications. The FC-V-50 polymer has a negative charge due to the presence of carboxyl functional groups, and the TZ-001 polymer has a positive charge due to the tertiary amine group at a pH of seven. In biosensing applications, non-specific adsorption due to opposite charges of biomolecules and microdisk surfaces can adversely affect the performance of the biosensor. By mixing FC-V-50 and TZ-001 polymers in different ratios, the microdisk surface charge was controlled, and the non-specific adsorption of bovine serum albumin and lysozyme was studied. In addition, the label-free biosensing of streptavidin was demonstrated using a blended polymer-based microdisk laser. This work reports, to the best of our knowledge, the first demonstration of a blended polymer microdisk laser for controlling the non-specific adsorption of biomolecules.
采用喷墨打印法制备了一种混合 FC-V-50 和 TZ-001 聚合物的微盘激光器，并将其用于生物传感应用。由于存在羧基官能团，FC-V-50 聚合物带负电荷，而 TZ-001 聚合物由于在 pH 值为七时带有叔胺基团而带正电荷。在生物传感应用中，由于生物分子和微盘表面的相反电荷，非特异性吸附会对生物传感器的性能产生不利影响。通过混合 FC-V-50 和 TZ-001 聚合物以不同的比例，可以控制微盘表面电荷，并研究牛血清白蛋白和溶菌酶的非特异性吸附。此外，还使用混合聚合物微盘激光器演示了链霉亲和素的无标记生物传感。据我们所知，这项工作首次展示了混合聚合物微盘激光器在控制生物分子非特异性吸附方面的应用。
