Opt Lett. 2021 Jan 15;46(2):372-375. doi: 10.1364/OL.411861.
Optical resolution photoacoustic microscopy (ORPAM) has demonstrated both high resolution and rich contrast imaging of optical chromophores in biologic tissues. To date, sensitivity remains a major challenge for ORPAM, which limits the capability of resolving biologic microvascular networks. In this study, we propose and evaluate a new ORPAM modality termed as optical resolution photoacoustic computed microscopy (ORPACM), through the combination of a two-dimensional laser-scanning system with a medical ultrasonographic platform. Apart from conventional ORPAMs, we record multiple photoacoustic (PA) signals using a 128-element ultrasonic transducer array for each pulse excitation. Then, we apply a reconstruction algorithm to recover one depth-resolved PA signal referred to as an A-line, which reveals more detailed information compared with conventional single-element transducer-based ORPAMs. In addition, we carried out both in vitro and in vivo experiments as well as quantitative analyses to show the advanced features of ORPACM.
光学解谐光声显微镜（ORPAM）已经证明了在生物组织中对光学色素的高分辨率和丰富对比度成像。迄今为止，灵敏度仍然是 ORPAM 的主要挑战，这限制了其分辨生物微血管网络的能力。在这项研究中，我们通过将二维激光扫描系统与医学超声平台相结合，提出并评估了一种称为光学解谐光声计算显微镜（ORPACM）的新 ORPAM 模式。除了传统的 ORPAMs 之外，我们还使用 128 元件超声换能器阵列为每个脉冲激励记录多个光声（PA）信号。然后，我们应用重建算法来恢复一个深度分辨的 PA 信号，称为 A 线，与传统的基于单元素换能器的 ORPAMs 相比，它可以提供更详细的信息。此外，我们进行了体外和体内实验以及定量分析，以展示 ORAPCM 的先进功能。
