Zhuang Zhou, John Johnson V, Liao Haofu, Luo Jiebo, Rubery Paul, Mesfin Addisu, Boda Sunil Kumar, Xie Jingwei, Zhang Xinping
Center for Musculoskeletal Research, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14621, United States.
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska, Omaha, Nebraska 68198, United States.
ACS Biomater Sci Eng. 2020 Nov 9;6(11):6241-6252. doi: 10.1021/acsbiomaterials.0c00421. Epub 2020 Oct 22.
Structural bone allograft transplantation remains one of the common strategies for repair and reconstruction of large bone defects. Due to the loss of periosteum that covers the outer surface of the cortical bone, the healing and incorporation of allografts is extremely slow and limited. To enhance the biological performance of allografts, herein, we report a novel and simple approach for engineering a periosteum mimetic coating on the surface of structural bone allografts polymer-mediated electrospray deposition. This approach enables the coating on allografts with precisely controlled composition and thickness. In addition, the periosteum mimetic coating can be tailored to achieve desired drug release profiles by making use of an appropriate biodegradable polymer or polymer blend. The efficacy study in a murine segmental femoral bone defect model demonstrates that the allograft coating composed of poly(lactic--glycolic acid) and bone morphogenetic protein-2 mimicking peptide significantly improves allograft healing as evidenced by decreased fibrotic tissue formation, increased periosteal bone formation, and enhanced osseointegration. Taken together, this study provides a platform technology for engineering a periosteum mimetic coating which can greatly promote bone allograft healing. This technology could eventually result in an off-the-shelf and multifunctional structural bone allograft for highly effective repair and reconstruction of large segmental bone defects. The technology can also be used to ameliorate the performance of other medical implants by modifying their surfaces.
结构骨移植仍然是修复和重建大骨缺损的常见策略之一。由于覆盖皮质骨外表面的骨膜缺失，同种异体移植物的愈合和整合极其缓慢且有限。为了提高同种异体移植物的生物学性能，在此，我们报告了一种新颖且简单的方法，用于在结构骨同种异体移植物表面构建仿骨膜涂层——聚合物介导的电喷雾沉积法。这种方法能够在同种异体移植物上形成成分和厚度精确可控的涂层。此外，通过使用合适的可生物降解聚合物或聚合物共混物，可以对仿骨膜涂层进行定制，以实现所需的药物释放曲线。在小鼠节段性股骨缺损模型中的功效研究表明，由聚（乳酸 - 乙醇酸）和骨形态发生蛋白 -2 模拟肽组成的同种异体移植物涂层显著改善了同种异体移植物的愈合，表现为纤维化组织形成减少、骨膜骨形成增加以及骨整合增强。综上所述，本研究提供了一种用于构建仿骨膜涂层的平台技术，该技术可极大地促进骨同种异体移植物的愈合。这项技术最终可能会产生一种现成的多功能结构骨同种异体移植物，用于高效修复和重建大节段骨缺损。该技术还可用于通过修饰其他医疗植入物的表面来改善其性能。
